PMID- 16395335
OWN - NLM
STAT- MEDLINE
DCOM- 20060307
LR  - 20190816
IS  - 0261-4189 (Print)
IS  - 1460-2075 (Electronic)
IS  - 0261-4189 (Linking)
VI  - 25
IP  - 1
DP  - 2006 Jan 11
TI  - Interplay between human DNA repair proteins at a unique double-strand break in 
      vivo.
PG  - 222-31
AB  - DNA repair by homologous recombination is essential for preserving genomic 
      integrity. The RAD51 paralogs (RAD51B, RAD51C, RAD51D, XRCC2 and XRCC3) play 
      important roles in this process. In this study, we show that human RAD51 
      interacts with RAD51C-XRCC3 or RAD51B-C-D-XRCC2. In addition to being critical 
      for RAD51 focus formation, RAD51C localizes to DNA damage sites. Inhibition of 
      RAD51C results in a decrease in cellular proliferation consistent with a role in 
      repairing double-strand breaks (DSBs) that occur naturally. To monitor a single 
      DNA repair event, we developed immunofluorescence and chromatin 
      immunoprecipitation (ChIP) methods on human cells where a unique DSB can be 
      created in vivo. Using this system, we observed a single focus of RAD51C, RAD51 
      and 53BP1, which colocalized with gamma-H2AX. ChIPs revealed that endogenous 
      human RAD51, RAD51C, RAD51D, XRCC2, XRCC3 and MRE11 proteins are recruited in the 
      S-G2 phase of the cell cycle, while Ku80 is recruited during G1. We propose that 
      RAD51C ensures a tight regulation of RAD51 assembly during DSB repair and plays a 
      direct role in repairing DSBs in vivo.
FAU - Rodrigue, Amelie
AU  - Rodrigue A
AD  - Genome Stability Laboratory, Laval University Cancer Research Center, Quebec 
      City, Quebec, Canada.
FAU - Lafrance, Matthieu
AU  - Lafrance M
FAU - Gauthier, Marie-Christine
AU  - Gauthier MC
FAU - McDonald, Darin
AU  - McDonald D
FAU - Hendzel, Michael
AU  - Hendzel M
FAU - West, Stephen C
AU  - West SC
FAU - Jasin, Maria
AU  - Jasin M
FAU - Masson, Jean-Yves
AU  - Masson JY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060105
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (H2AX protein, human)
RN  - 0 (Histones)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (RAD51C protein, human)
RN  - 0 (TP53BP1 protein, human)
RN  - 0 (Tumor Suppressor p53-Binding Protein 1)
RN  - 0 (X-ray repair cross complementing protein 3)
RN  - 0 (XRCC2 protein, human)
RN  - 9007-49-2 (DNA)
RN  - EC 2.7.7.- (RAD51 protein, human)
RN  - EC 2.7.7.- (Rad51 Recombinase)
SB  - IM
MH  - Cell Cycle
MH  - Cells, Cultured
MH  - Chromatin Immunoprecipitation
MH  - DNA/chemistry/metabolism
MH  - *DNA Damage
MH  - *DNA Repair
MH  - DNA-Binding Proteins/analysis/antagonists & inhibitors/genetics/*metabolism
MH  - Histones/analysis/metabolism
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins/analysis/metabolism
MH  - Phosphoproteins/analysis/metabolism
MH  - Rad51 Recombinase/analysis/genetics/*metabolism
MH  - *Recombination, Genetic
MH  - Tumor Suppressor p53-Binding Protein 1
PMC - PMC1356357
EDAT- 2006/01/06 09:00
MHDA- 2006/03/08 09:00
PMCR- 2007/01/11
CRDT- 2006/01/06 09:00
PHST- 2005/08/11 00:00 [received]
PHST- 2005/11/22 00:00 [accepted]
PHST- 2006/01/06 09:00 [pubmed]
PHST- 2006/03/08 09:00 [medline]
PHST- 2006/01/06 09:00 [entrez]
PHST- 2007/01/11 00:00 [pmc-release]
AID - 7600914 [pii]
AID - 10.1038/sj.emboj.7600914 [doi]
PST - ppublish
SO  - EMBO J. 2006 Jan 11;25(1):222-31. doi: 10.1038/sj.emboj.7600914. Epub 2006 Jan 5.