PMID- 16397499
OWN - NLM
STAT- MEDLINE
DCOM- 20060124
LR  - 20220812
IS  - 1476-4687 (Electronic)
IS  - 0028-0836 (Linking)
VI  - 439
IP  - 7072
DP  - 2006 Jan 5
TI  - Generation of a functional mammary gland from a single stem cell.
PG  - 84-8
AB  - The existence of mammary stem cells (MaSCs) has been postulated from evidence 
      that the mammary gland can be regenerated by transplantation of epithelial 
      fragments in mice. Interest in MaSCs has been further stimulated by their 
      potential role in breast tumorigenesis. However, the identity and purification of 
      MaSCs has proved elusive owing to the lack of defined markers. We isolated 
      discrete populations of mouse mammary cells on the basis of cell-surface markers 
      and identified a subpopulation (Lin-CD29hiCD24+) that is highly enriched for 
      MaSCs by transplantation. Here we show that a single cell, marked with a LacZ 
      transgene, can reconstitute a complete mammary gland in vivo. The transplanted 
      cell contributed to both the luminal and myoepithelial lineages and generated 
      functional lobuloalveolar units during pregnancy. The self-renewing capacity of 
      these cells was demonstrated by serial transplantation of clonal outgrowths. In 
      support of a potential role for MaSCs in breast cancer, the stem-cell-enriched 
      subpopulation was expanded in premalignant mammary tissue from MMTV-wnt-1 mice 
      and contained a higher number of MaSCs. Our data establish that single cells 
      within the Lin-CD29hiCD24+ population are multipotent and self-renewing, 
      properties that define them as MaSCs.
FAU - Shackleton, Mark
AU  - Shackleton M
AD  - The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, 
      Parkville, Victoria 3050, Australia.
FAU - Vaillant, Francois
AU  - Vaillant F
FAU - Simpson, Kaylene J
AU  - Simpson KJ
FAU - Stingl, John
AU  - Stingl J
FAU - Smyth, Gordon K
AU  - Smyth GK
FAU - Asselin-Labat, Marie-Liesse
AU  - Asselin-Labat ML
FAU - Wu, Li
AU  - Wu L
FAU - Lindeman, Geoffrey J
AU  - Lindeman GJ
FAU - Visvader, Jane E
AU  - Visvader JE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nature
JT  - Nature
JID - 0410462
RN  - 0 (CD24 Antigen)
RN  - 0 (Integrin beta1)
RN  - 68238-35-7 (Keratins)
SB  - IM
MH  - Animals
MH  - CD24 Antigen/metabolism
MH  - Cell Differentiation
MH  - Cell Division
MH  - Epithelial Cells/cytology/metabolism
MH  - Female
MH  - Genes, Reporter/genetics
MH  - Integrin beta1/metabolism
MH  - Keratins/metabolism
MH  - Lac Operon/genetics
MH  - Mammary Glands, Animal/*cytology/*physiology
MH  - Mice
MH  - Mice, Transgenic
MH  - Multipotent Stem Cells/*cytology/metabolism
MH  - Regeneration/*physiology
MH  - Stem Cell Transplantation
EDAT- 2006/01/07 09:00
MHDA- 2006/01/25 09:00
CRDT- 2006/01/07 09:00
PHST- 2005/08/01 00:00 [received]
PHST- 2005/10/13 00:00 [accepted]
PHST- 2006/01/07 09:00 [pubmed]
PHST- 2006/01/25 09:00 [medline]
PHST- 2006/01/07 09:00 [entrez]
AID - nature04372 [pii]
AID - 10.1038/nature04372 [doi]
PST - ppublish
SO  - Nature. 2006 Jan 5;439(7072):84-8. doi: 10.1038/nature04372.