PMID- 1644218
OWN - NLM
STAT- MEDLINE
DCOM- 19920909
LR  - 20190721
IS  - 0012-1606 (Print)
IS  - 0012-1606 (Linking)
VI  - 152
IP  - 2
DP  - 1992 Aug
TI  - Tetraploidy in mice, embryonic cell number, and the grain of the developmental 
      map.
PG  - 233-41
AB  - Tetraploid mice prepared by electrofusion develop for up to 14 days in utero. The 
      embryos are essentially normal save that the forebrain and its associated tissues 
      fail to develop properly. Here, we report measurements of cell counts in tissues 
      and volume measurements of tetraploid and control embryos together with 
      observations on the morphology of tetraploid embryos. The results show that the 
      tetraploid embryos are about 85% normal size, but have only a little under half 
      the number of cells of control embryos, with their nuclei being about twice the 
      size of those of diploid cells. Close examination of sectioned material, in 
      contrast, showed that tetraploid morphology and morphogenesis were 
      indistinguishable from those of controls, except in forebrain-associated 
      material. This conclusion gives some insight into an important developmental 
      question, how fine can the developmental map be for normal cellular 
      differentiation to proceed? As tetraploids have only about half the expected 
      number of cells, the ability of these embryos to develop normally in all regions 
      except the forebrain and its derivatives argues that pattern formation mechanisms 
      can cope with the abnormally small number of cells in all regions except the 
      forebrain. The results as a whole argue for size regulation in mammalian embryos 
      being achieved by assaying absolute size rather than counting cell numbers.
FAU - Henery, C C
AU  - Henery CC
AD  - Department of Anatomy, Edinburgh University, U.K.
FAU - Bard, J B
AU  - Bard JB
FAU - Kaufman, M H
AU  - Kaufman MH
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Dev Biol
JT  - Developmental biology
JID - 0372762
SB  - IM
MH  - Animals
MH  - Cell Count
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred CBA
MH  - Morphogenesis
MH  - *Polyploidy
MH  - Prosencephalon/abnormalities/*embryology
EDAT- 1992/08/01 00:00
MHDA- 1992/08/01 00:01
CRDT- 1992/08/01 00:00
PHST- 1992/08/01 00:00 [pubmed]
PHST- 1992/08/01 00:01 [medline]
PHST- 1992/08/01 00:00 [entrez]
AID - 0012-1606(92)90131-Y [pii]
AID - 10.1016/0012-1606(92)90131-y [doi]
PST - ppublish
SO  - Dev Biol. 1992 Aug;152(2):233-41. doi: 10.1016/0012-1606(92)90131-y.