PMID- 1661734
OWN - NLM
STAT- MEDLINE
DCOM- 19920205
LR  - 20190508
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 115
IP  - 6
DP  - 1991 Dec
TI  - A cell culture model of the blood-brain barrier.
PG  - 1725-35
AB  - Endothelial cells that make up brain capillaries and constitute the blood-brain 
      barrier become different from peripheral endothelial cells in response to 
      inductive factors found in the nervous system. We have established a cell culture 
      model of the blood-brain barrier by treating brain endothelial cells with a 
      combination of astrocyte-conditioned medium and agents that elevate intracellular 
      cAMP. These cells form high resistance tight junctions and exhibit low rates of 
      paracellular leakage and fluid-phase endocytosis. They also undergo a dramatic 
      structural reorganization as they form tight junctions. Results from these 
      studies suggest modes of manipulating the permeability of the blood-brain 
      barrier, potentially providing the basis for increasing the penetration of drugs 
      into the central nervous system.
FAU - Rubin, L L
AU  - Rubin LL
AD  - Athena Neurosciences, Inc., South San Francisco, California 94080.
FAU - Hall, D E
AU  - Hall DE
FAU - Porter, S
AU  - Porter S
FAU - Barbu, K
AU  - Barbu K
FAU - Cannon, C
AU  - Cannon C
FAU - Horner, H C
AU  - Horner HC
FAU - Janatpour, M
AU  - Janatpour M
FAU - Liaw, C W
AU  - Liaw CW
FAU - Manning, K
AU  - Manning K
FAU - Morales, J
AU  - Morales J
AU  - et al.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Culture Media)
RN  - E0399OZS9N (Cyclic AMP)
RN  - H2D2X058MU (Cyclic GMP)
SB  - IM
MH  - Animals
MH  - Astrocytes/cytology/metabolism
MH  - Biological Transport
MH  - *Blood-Brain Barrier
MH  - Cattle
MH  - Cells, Cultured
MH  - Clone Cells
MH  - Culture Media
MH  - Cyclic AMP/metabolism
MH  - Cyclic GMP/metabolism
MH  - Endocytosis
MH  - Endothelium/cytology/metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Intercellular Junctions/metabolism
MH  - Kinetics
MH  - *Models, Biological
MH  - Rats
PMC - PMC2289219
EDAT- 1991/12/01 00:00
MHDA- 1991/12/01 00:01
PMCR- 1992/06/02
CRDT- 1991/12/01 00:00
PHST- 1991/12/01 00:00 [pubmed]
PHST- 1991/12/01 00:01 [medline]
PHST- 1991/12/01 00:00 [entrez]
PHST- 1992/06/02 00:00 [pmc-release]
AID - 92098584 [pii]
AID - 10.1083/jcb.115.6.1725 [doi]
PST - ppublish
SO  - J Cell Biol. 1991 Dec;115(6):1725-35. doi: 10.1083/jcb.115.6.1725.