PMID- 16702421
OWN - NLM
STAT- MEDLINE
DCOM- 20061127
LR  - 20181113
IS  - 0016-6731 (Print)
IS  - 0016-6731 (Linking)
VI  - 173
IP  - 3
DP  - 2006 Jul
TI  - Developmental modulation of nonhomologous end joining in Caenorhabditis elegans.
PG  - 1301-17
AB  - Homologous recombination and nonhomologous end joining (NHEJ) are important DNA 
      double-strand break repair pathways in many organisms. C. elegans strains 
      harboring mutations in the cku-70, cku-80, or lig-4 NHEJ genes displayed multiple 
      developmental abnormalities in response to radiation-induced DNA damage in 
      noncycling somatic cells. These phenotypes did not result from S-phase, DNA 
      damage, or mitotic checkpoints, apoptosis, or stress response pathways that 
      regulate dauer formation. However, an additional defect in him-10, a kinetochore 
      component, synergized with NHEJ mutations for the radiation-induced developmental 
      phenotypes, suggesting that they may be triggered by mis-segregation of 
      chromosome fragments. Although NHEJ was an important DNA repair pathway for 
      noncycling somatic cells in C. elegans, homologous recombination was used to 
      repair radiation-induced DNA damage in cycling somatic cells and in germ cells at 
      all times. Noncycling germ cells that depended on homologous recombination 
      underwent cell cycle arrest in G2, whereas noncycling somatic cells that depended 
      on NHEJ arrested in G1, suggesting that cell cycle phase may modulate DNA repair 
      during development. We conclude that error-prone NHEJ plays little or no role in 
      DNA repair in C. elegans germ cells, possibly ensuring homology-based 
      double-strand break repair and transmission of a stable genome from one 
      generation to the next.
FAU - Clejan, Iuval
AU  - Clejan I
AD  - Department of Genetics, University of North Carolina, Chapel Hill, North Carolina 
      27599-3280, USA.
FAU - Boerckel, Julie
AU  - Boerckel J
FAU - Ahmed, Shawn
AU  - Ahmed S
LA  - eng
GR  - R01 GM066228/GM/NIGMS NIH HHS/United States
GR  - R56 GM066228/GM/NIGMS NIH HHS/United States
GR  - GM066228/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20060515
PL  - United States
TA  - Genetics
JT  - Genetics
JID - 0374636
SB  - IM
MH  - Animals
MH  - Caenorhabditis elegans/*genetics/*growth & development
MH  - *DNA Repair
MH  - *Gene Expression Regulation, Developmental/physiology
MH  - Germ Cells
MH  - Kinetochores
MH  - Larva
MH  - Models, Biological
MH  - Models, Genetic
MH  - Mutation
MH  - Phenotype
MH  - *Recombination, Genetic
PMC - PMC1526663
EDAT- 2006/05/17 09:00
MHDA- 2006/12/09 09:00
PMCR- 2006/10/01
CRDT- 2006/05/17 09:00
PHST- 2006/05/17 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2006/05/17 09:00 [entrez]
PHST- 2006/10/01 00:00 [pmc-release]
AID - genetics.106.058628 [pii]
AID - gen17331301 [pii]
AID - 10.1534/genetics.106.058628 [doi]
PST - ppublish
SO  - Genetics. 2006 Jul;173(3):1301-17. doi: 10.1534/genetics.106.058628. Epub 2006 
      May 15.