PMID- 1693434
OWN - NLM
STAT- MEDLINE
DCOM- 19900712
LR  - 20240109
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 87
IP  - 11
DP  - 1990 Jun
TI  - Anti-synthetic peptide antibody reacting at the fusion junction of 
      deletion-mutant epidermal growth factor receptors in human glioblastoma.
PG  - 4207-11
AB  - We have investigated human gliomas that amplify and rearrange the epidermal 
      growth factor receptor gene, with generation of an in-frame deletion mutation of 
      802 nucleotides in the external domain. This in-frame deletion mutation generates 
      a local amino acid sequence at the fusion junction of what normally were distant 
      polypeptide sequences in the intact epidermal growth factor receptor. This 
      14-amino acid peptide was chemically synthesized, coupled to keyhole limpet 
      hemocyanin, and used as an immunogen in rabbits. The elicited antibody reacted 
      specifically with the fusion peptide in ELISA. The anti-fusion junction peptide 
      antibody was purified by passage of the antiserum over a peptide affinity column 
      with acidic elution. The purified antibody selectively bound the glioma deletion 
      mutant as compared to the intact epidermal growth factor receptor as assessed by 
      immunocytochemistry, immunofluorescence, immunoprecipitation with gel 
      electrophoresis, and binding experiments using radioiodinated antibody. These 
      data indicate that it is feasible to generate site-specific anti-peptide 
      antibodies that are highly selective for mutant proteins in human tumors. The 
      anti-peptide antibody described here, and other mutation site-specific 
      antibodies, should be ideal candidates for tumor immunoimaging and immunotherapy.
FAU - Humphrey, P A
AU  - Humphrey PA
AD  - Department of Pathology, Duke University Medical Center, Durham, NC 27710.
FAU - Wong, A J
AU  - Wong AJ
FAU - Vogelstein, B
AU  - Vogelstein B
FAU - Zalutsky, M R
AU  - Zalutsky MR
FAU - Fuller, G N
AU  - Fuller GN
FAU - Archer, G E
AU  - Archer GE
FAU - Friedman, H S
AU  - Friedman HS
FAU - Kwatra, M M
AU  - Kwatra MM
FAU - Bigner, S H
AU  - Bigner SH
FAU - Bigner, D D
AU  - Bigner DD
LA  - eng
GR  - CA11898/CA/NCI NIH HHS/United States
GR  - NS-20023-05/NS/NINDS NIH HHS/United States
GR  - T32-NS07304/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Antibodies)
RN  - 0 (Epitopes)
RN  - 0 (Peptides)
RN  - EC 2.7.10.1 (ErbB Receptors)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antibodies/*immunology/isolation & purification
MH  - Base Sequence
MH  - Cell Membrane/metabolism
MH  - Endocytosis
MH  - Epitopes
MH  - ErbB Receptors/genetics/*immunology/metabolism
MH  - Glioma/metabolism
MH  - Humans
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Peptides/immunology
MH  - Precipitin Tests
MH  - Protein-Tyrosine Kinases/metabolism
PMC - PMC54077
EDAT- 1990/06/01 00:00
MHDA- 1990/06/01 00:01
PMCR- 1990/12/01
CRDT- 1990/06/01 00:00
PHST- 1990/06/01 00:00 [pubmed]
PHST- 1990/06/01 00:01 [medline]
PHST- 1990/06/01 00:00 [entrez]
PHST- 1990/12/01 00:00 [pmc-release]
AID - 10.1073/pnas.87.11.4207 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1990 Jun;87(11):4207-11. doi: 10.1073/pnas.87.11.4207.