PMID- 16957781
OWN - NLM
STAT- MEDLINE
DCOM- 20061024
LR  - 20231105
IS  - 0261-4189 (Print)
IS  - 1460-2075 (Electronic)
IS  - 0261-4189 (Linking)
VI  - 25
IP  - 18
DP  - 2006 Sep 20
TI  - New functions of XPC in the protection of human skin cells from oxidative damage.
PG  - 4305-15
AB  - Xeroderma pigmentosum (XP) C is involved in the recognition of a variety of bulky 
      DNA-distorting lesions in nucleotide excision repair. Here, we show that XPC 
      plays an unexpected and multifaceted role in cell protection from oxidative DNA 
      damage. XP-C primary keratinocytes and fibroblasts are hypersensitive to the 
      killing effects of DNA-oxidizing agents and this effect is reverted by expression 
      of wild-type XPC. Upon oxidant exposure, XP-C primary keratinocytes and 
      fibroblasts accumulate 8,5'-cyclopurine 2'-deoxynucleosides in their DNA, 
      indicating that XPC is involved in their removal. In the absence of XPC, a 
      decrease in the repair rate of 8-hydroxyguanine (8-OH-Gua) is also observed. We 
      demonstrate that XPC-HR23B complex acts as cofactor in base excision repair of 
      8-OH-Gua, by stimulating the activity of its specific DNA glycosylase OGG1. In 
      vitro experiments suggest that the mechanism involved is a combination of 
      increased loading and turnover of OGG1 by XPC-HR23B complex. The accumulation of 
      endogenous oxidative DNA damage might contribute to increased skin cancer risk 
      and account for internal cancers reported for XP-C patients.
FAU - D'Errico, Mariarosaria
AU  - D'Errico M
AD  - Department of Environment and Primary Prevention, Istituto Superiore di Sanita, 
      Rome, Italy.
FAU - Parlanti, Eleonora
AU  - Parlanti E
FAU - Teson, Massimo
AU  - Teson M
FAU - de Jesus, Bruno M Bernardes
AU  - de Jesus BM
FAU - Degan, Paolo
AU  - Degan P
FAU - Calcagnile, Angelo
AU  - Calcagnile A
FAU - Jaruga, Pawel
AU  - Jaruga P
FAU - Bjoras, Magnar
AU  - Bjoras M
FAU - Crescenzi, Marco
AU  - Crescenzi M
FAU - Pedrini, Antonia M
AU  - Pedrini AM
FAU - Egly, Jean-Marc
AU  - Egly JM
FAU - Zambruno, Giovanna
AU  - Zambruno G
FAU - Stefanini, Miria
AU  - Stefanini M
FAU - Dizdaroglu, Miral
AU  - Dizdaroglu M
FAU - Dogliotti, Eugenia
AU  - Dogliotti E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060907
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Bromates)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Oxidants)
RN  - 04MB35W6ZA (potassium bromate)
RN  - 156533-33-4 (RAD23A protein, human)
RN  - 156533-34-5 (XPC protein, human)
RN  - 5614-64-2 (8-hydroxyguanine)
RN  - 5Z93L87A1R (Guanine)
RN  - EC 3.2.2.- (DNA Glycosylases)
RN  - EC 3.2.2.- (oxoguanine glycosylase 1, human)
RN  - EC 6.5.1.- (DNA Repair Enzymes)
SB  - IM
MH  - Bromates/toxicity
MH  - Cells, Cultured
MH  - *DNA Damage
MH  - DNA Glycosylases/metabolism
MH  - DNA Repair
MH  - DNA Repair Enzymes
MH  - DNA-Binding Proteins/deficiency/genetics/*metabolism
MH  - Guanine/analogs & derivatives/metabolism
MH  - Humans
MH  - Keratinocytes/drug effects/*metabolism/radiation effects
MH  - Oxidants/toxicity
MH  - Skin Neoplasms/etiology
MH  - X-Rays
MH  - Xeroderma Pigmentosum/complications/genetics/metabolism
PMC - PMC1570445
EDAT- 2006/09/08 09:00
MHDA- 2006/10/25 09:00
PMCR- 2007/09/20
CRDT- 2006/09/08 09:00
PHST- 2006/02/06 00:00 [received]
PHST- 2006/07/12 00:00 [accepted]
PHST- 2006/09/08 09:00 [pubmed]
PHST- 2006/10/25 09:00 [medline]
PHST- 2006/09/08 09:00 [entrez]
PHST- 2007/09/20 00:00 [pmc-release]
AID - 7601277 [pii]
AID - 10.1038/sj.emboj.7601277 [doi]
PST - ppublish
SO  - EMBO J. 2006 Sep 20;25(18):4305-15. doi: 10.1038/sj.emboj.7601277. Epub 2006 Sep 
      7.