PMID- 17132723
OWN - NLM
STAT- MEDLINE
DCOM- 20070531
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 1528-0020 (Electronic)
IS  - 0006-4971 (Linking)
VI  - 109
IP  - 7
DP  - 2007 Apr 1
TI  - HIV-1 infection and pathogenesis in a novel humanized mouse model.
PG  - 2978-81
AB  - The Rag2-gammaC double-knockout (DKO) mouse lacks T, B, and natural killer (NK) 
      cells, and allows development of a functional human immune system with human 
      CD34+ hematopoietic stem/progenitor cells (DKO-hu HSCs). Normal human T, B, and 
      dendritic cells are present in peripheral blood, thymus, spleen, and lymph nodes. 
      We report that both CCR5 and CXCR4 are expressed on human immature and mature T 
      cells. DKO-hu HSC mice allow efficient HIV-1 infection with plasma high viremia. 
      High levels of productive infection occur in the thymus, spleen, and lymph nodes. 
      Human CD4+ T cells are gradually depleted by HIV-1 in a dose-dependent manner. In 
      addition, HIV-1 infection persists in infected DKO-hu HSC mice for at least 19 
      weeks, with infectious HIV-1 in lymphoid tissues. Thus, the DKO-hu HSC mouse can 
      serve as a relevant in vivo model to investigate mechanisms of HIV-1 infection 
      and immunopathogenesis as well as to develop anti-HIV-1 therapeutics.
FAU - Zhang, Liguo
AU  - Zhang L
AD  - Department of Microbiology and Immunology, The Lineberger Comprehensive Cancer 
      Center, University of North Carolina, Chapel Hill 27599-7295, USA.
FAU - Kovalev, Grigoriy I
AU  - Kovalev GI
FAU - Su, Lishan
AU  - Su L
LA  - eng
GR  - AI/GM 48407/AI/NIAID NIH HHS/United States
GR  - AI041356/AI/NIAID NIH HHS/United States
GR  - CA99939/CA/NCI NIH HHS/United States
GR  - P30 AI50410/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Rag2 protein, mouse)
SB  - IM
MH  - Animals
MH  - DNA-Binding Proteins/deficiency/genetics
MH  - Disease Models, Animal
MH  - HIV Infections/*etiology/*immunology/virology
MH  - *HIV-1/physiology
MH  - Humans
MH  - Mice
MH  - Mice, Knockout
MH  - Transplantation Chimera
MH  - Virus Replication
PMC - PMC1852218
EDAT- 2006/11/30 09:00
MHDA- 2007/06/01 09:00
PMCR- 2008/04/01
CRDT- 2006/11/30 09:00
PHST- 2006/11/30 09:00 [pubmed]
PHST- 2007/06/01 09:00 [medline]
PHST- 2006/11/30 09:00 [entrez]
PHST- 2008/04/01 00:00 [pmc-release]
AID - S0006-4971(20)41802-6 [pii]
AID - 2006/033159 [pii]
AID - 10.1182/blood-2006-07-033159 [doi]
PST - ppublish
SO  - Blood. 2007 Apr 1;109(7):2978-81. doi: 10.1182/blood-2006-07-033159.