PMID- 17918903
OWN - NLM
STAT- MEDLINE
DCOM- 20080109
LR  - 20220309
IS  - 1530-6984 (Print)
IS  - 1530-6984 (Linking)
VI  - 7
IP  - 11
DP  - 2007 Nov
TI  - Active nanodiamond hydrogels for chemotherapeutic delivery.
PG  - 3305-14
AB  - Nanodiamond materials can serve as highly versatile platforms for the controlled 
      functionalization and delivery of a wide spectrum of therapeutic elements. In 
      this work, doxorubicin hydrochloride (DOX), an apoptosis-inducing drug widely 
      used in chemotherapy, was successfully applied toward the functionalization of 
      nanodiamond materials (NDs, 2-8 nm) and introduced toward murine macrophages as 
      well as human colorectal carcinoma cells with preserved efficacy. The adsorption 
      of DOX onto the NDs and its reversible release were achieved by regulating Cl- 
      ion concentration, and the NDs were found to be able to efficiently ferry the 
      drug inside living cells. Comprehensive bioassays were performed to assess and 
      confirm the innate biocompatibility of the NDs, via real-time quantitative 
      polymerase chain reaction (RT-PCR), and electrophoretic DNA fragmentation as well 
      as MTT analysis confirmed the functional apoptosis-inducing mechanisms driven by 
      the DOX-functionalized NDs. We extended the applicability of the DOX-ND 
      composites toward a translational context, where MTT assays were performed on the 
      HT-29 colon cancer cell line to assess DOX-ND induced cell death and ND-mediated 
      chemotherapeutic sequestering for potential slow/sustained released capabilities. 
      These and other medically relevant capabilities enabled by the NDs forge its 
      strong potential as a therapeutically significant nanomaterial.
FAU - Huang, Houjin
AU  - Huang H
AD  - Department of Biomedical Engineering, Robert R. McCormick School of Engineering 
      and Applied Science, Northwestern University, Evanston, Illinois 60208, USA.
FAU - Pierstorff, Erik
AU  - Pierstorff E
FAU - Osawa, Eiji
AU  - Osawa E
FAU - Ho, Dean
AU  - Ho D
LA  - eng
GR  - U54 A1065359/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20071005
PL  - United States
TA  - Nano Lett
JT  - Nano letters
JID - 101088070
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Biocompatible Materials)
RN  - 0 (Tetrazolium Salts)
RN  - 0 (Thiazoles)
RN  - 7782-40-3 (Diamond)
RN  - 80168379AG (Doxorubicin)
RN  - EUY85H477I (thiazolyl blue)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents/*administration & dosage
MH  - Apoptosis
MH  - Biocompatible Materials
MH  - Cell Line, Tumor
MH  - Colonic Neoplasms/drug therapy
MH  - *Diamond
MH  - Doxorubicin/administration & dosage
MH  - *Drug Delivery Systems
MH  - Humans
MH  - Macrophages/drug effects
MH  - Mice
MH  - Nanoparticles/*chemistry
MH  - Nanotechnology/*methods
MH  - Neoplasms/*drug therapy
MH  - Spectrophotometry, Ultraviolet/methods
MH  - Tetrazolium Salts/pharmacology
MH  - Thiazoles/pharmacology
EDAT- 2007/10/09 09:00
MHDA- 2008/01/10 09:00
CRDT- 2007/10/09 09:00
PHST- 2007/10/09 09:00 [pubmed]
PHST- 2008/01/10 09:00 [medline]
PHST- 2007/10/09 09:00 [entrez]
AID - 10.1021/nl071521o [doi]
PST - ppublish
SO  - Nano Lett. 2007 Nov;7(11):3305-14. doi: 10.1021/nl071521o. Epub 2007 Oct 5.