PMID- 17934449
OWN - NLM
STAT- MEDLINE
DCOM- 20071126
LR  - 20250214
IS  - 1476-4687 (Electronic)
IS  - 0028-0836 (Linking)
VI  - 449
IP  - 7165
DP  - 2007 Oct 25
TI  - Identification of stem cells in small intestine and colon by marker gene Lgr5.
PG  - 1003-7
AB  - The intestinal epithelium is the most rapidly self-renewing tissue in adult 
      mammals. It is currently believed that four to six crypt stem cells reside at the 
      +4 position immediately above the Paneth cells in the small intestine; colon stem 
      cells remain undefined. Lgr5 (leucine-rich-repeat-containing G-protein-coupled 
      receptor 5, also known as Gpr49) was selected from a panel of intestinal Wnt 
      target genes for its restricted crypt expression. Here, using two knock-in 
      alleles, we reveal exclusive expression of Lgr5 in cycling columnar cells at the 
      crypt base. In addition, Lgr5 was expressed in rare cells in several other 
      tissues. Using an inducible Cre knock-in allele and the Rosa26-lacZ reporter 
      strain, lineage-tracing experiments were performed in adult mice. The 
      Lgr5-positive crypt base columnar cell generated all epithelial lineages over a 
      60-day period, suggesting that it represents the stem cell of the small intestine 
      and colon. The expression pattern of Lgr5 suggests that it marks stem cells in 
      multiple adult tissues and cancers.
FAU - Barker, Nick
AU  - Barker N
AD  - Hubrecht Institute, Uppsalalaan 8, 3584CT Utrecht, The Netherlands.
FAU - van Es, Johan H
AU  - van Es JH
FAU - Kuipers, Jeroen
AU  - Kuipers J
FAU - Kujala, Pekka
AU  - Kujala P
FAU - van den Born, Maaike
AU  - van den Born M
FAU - Cozijnsen, Miranda
AU  - Cozijnsen M
FAU - Haegebarth, Andrea
AU  - Haegebarth A
FAU - Korving, Jeroen
AU  - Korving J
FAU - Begthel, Harry
AU  - Begthel H
FAU - Peters, Peter J
AU  - Peters PJ
FAU - Clevers, Hans
AU  - Clevers H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071014
PL  - England
TA  - Nature
JT  - Nature
JID - 0410462
RN  - 0 (Biomarkers)
RN  - 0 (LGR5 protein, human)
RN  - 0 (Lgr5 protein, mouse)
RN  - 0 (Receptors, G-Protein-Coupled)
SB  - IM
MH  - Alleles
MH  - Animals
MH  - Biomarkers
MH  - Cell Line, Tumor
MH  - Colon/*cytology
MH  - Gene Expression Profiling
MH  - Genes, Reporter
MH  - Humans
MH  - Intestine, Small/*cytology
MH  - Mice
MH  - Paneth Cells/metabolism
MH  - Receptors, G-Protein-Coupled/genetics/*metabolism
MH  - Stem Cells/*metabolism
EDAT- 2007/10/16 09:00
MHDA- 2007/12/06 09:00
CRDT- 2007/10/16 09:00
PHST- 2007/06/21 00:00 [received]
PHST- 2007/08/24 00:00 [accepted]
PHST- 2007/10/16 09:00 [pubmed]
PHST- 2007/12/06 09:00 [medline]
PHST- 2007/10/16 09:00 [entrez]
AID - nature06196 [pii]
AID - 10.1038/nature06196 [doi]
PST - ppublish
SO  - Nature. 2007 Oct 25;449(7165):1003-7. doi: 10.1038/nature06196. Epub 2007 Oct 14.