PMID- 17951512
OWN - NLM
STAT- MEDLINE
DCOM- 20080521
LR  - 20211020
IS  - 1522-8517 (Print)
IS  - 1523-5866 (Electronic)
IS  - 1522-8517 (Linking)
VI  - 10
IP  - 1
DP  - 2008 Feb
TI  - Sensitive detection of human cytomegalovirus in tumors and peripheral blood of 
      patients diagnosed with glioblastoma.
PG  - 10-8
AB  - Human cytomegalovirus (HCMV) has been described to be associated with several 
      human malignancies, though the frequency of detection remains controversial. It 
      is unclear whether HCMV plays an active role in malignant tumor progression or 
      becomes reactivated under pathologic conditions that result in chronic 
      inflammation or immunosuppression. In this study, we report on the investigation 
      of detecting HCMV in the tumors and peripheral blood of patients with newly 
      diagnosed glioblastoma multiforme (GBM). Using immunohistochemistry, in situ 
      hybridization, and polymerase chain reaction amplification of viral DNA, the 
      detection of HCMV was investigated in tumor and blood specimens from patients 
      with GBM as well as in the peripheral blood of normal volunteers and patients 
      undergoing craniotomy for diagnoses other than GBM. We found that a high 
      percentage (>90%) of GBM tumors, not surrounding normal brain, are associated 
      with HCMV nucleic acids and proteins. Furthermore, a significant proportion of 
      patients (80%) with newly diagnosed GBM have detectable HCMV DNA in their 
      peripheral blood, while sero-positive normal donors and other surgical patients 
      did not exhibit detectable virus, suggesting either a systemic reactivation of 
      HCMV within patients with GBM or shedding of viral DNA from infected tumor cells 
      into the periphery. These results confirm the association of HCMV with malignant 
      gliomas and demonstrate that subclinical HCMV viremia (presence of viral DNA in 
      blood without clinical symptoms of infection) is a previously unrecognized 
      disease spectrum in patients with GBM.
FAU - Mitchell, Duane A
AU  - Mitchell DA
AD  - Duke University Medical Center, Division of Neurosurgery, Department of 
      Surgery,Durham, NC 27710, USA. d.mitchell@duke.edu
FAU - Xie, Weihua
AU  - Xie W
FAU - Schmittling, Robert
AU  - Schmittling R
FAU - Learn, Chris
AU  - Learn C
FAU - Friedman, Allan
AU  - Friedman A
FAU - McLendon, Roger E
AU  - McLendon RE
FAU - Sampson, John H
AU  - Sampson JH
LA  - eng
GR  - P50 CA108786/CA/NCI NIH HHS/United States
GR  - 1P50 CA108786 01/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20071019
PL  - England
TA  - Neuro Oncol
JT  - Neuro-oncology
JID - 100887420
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Brain Neoplasms/*virology
MH  - Cytomegalovirus/isolation & purification
MH  - Cytomegalovirus Infections/*complications/epidemiology
MH  - DNA, Viral/analysis
MH  - Female
MH  - Glioblastoma/*virology
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Viremia/complications/epidemiology
PMC - PMC2600830
EDAT- 2007/10/24 09:00
MHDA- 2008/05/22 09:00
PMCR- 2009/01/01
CRDT- 2007/10/24 09:00
PHST- 2007/10/24 09:00 [pubmed]
PHST- 2008/05/22 09:00 [medline]
PHST- 2007/10/24 09:00 [entrez]
PHST- 2009/01/01 00:00 [pmc-release]
AID - 15228517-2007-035 [pii]
AID - neu-10-1-10 [pii]
AID - 10.1215/15228517-2007-035 [doi]
PST - ppublish
SO  - Neuro Oncol. 2008 Feb;10(1):10-8. doi: 10.1215/15228517-2007-035. Epub 2007 Oct 
      19.