PMID- 18067886
OWN - NLM
STAT- MEDLINE
DCOM- 20080226
LR  - 20240312
IS  - 1095-564X (Electronic)
IS  - 0012-1606 (Print)
IS  - 0012-1606 (Linking)
VI  - 313
IP  - 2
DP  - 2008 Jan 15
TI  - Characterization of the Drosophila segment determination morphome.
PG  - 844-62
AB  - Here we characterize the expression of the full system of genes which control the 
      segmentation morphogenetic field of Drosophila at the protein level in one 
      dimension. The data used for this characterization are quantitative with cellular 
      resolution in space and about 6 min in time. We present the full quantitative 
      profiles of all 14 segmentation genes which act before the onset of gastrulation. 
      The expression patterns of these genes are first characterized in terms of their 
      average or typical behavior. At this level, the expression of all of the genes 
      has been integrated into a single atlas of gene expression in which the 
      expression levels of all genes in each cell are specified. We show that 
      expression domains do not arise synchronously, but rather each domain has its own 
      specific dynamics of formation. Moreover, we show that the expression domains 
      shift position in the direction of the cephalic furrow, such that domains in the 
      anlage of the segmented germ band shift anteriorly while those in the presumptive 
      head shift posteriorly. The expression atlas of integrated data is very close to 
      the expression profiles of individual embryos during the latter part of the 
      blastoderm stage. At earlier times gap gene domains show considerable variation 
      in amplitude, and significant positional variability. Nevertheless, an average 
      early gap domain is close to that of a median individual. In contrast, we show 
      that there is a diversity of developmental trajectories among pair-rule genes at 
      a variety of levels, including the order of domain formation and positional 
      accuracy. We further show that this variation is dynamically reduced, or 
      canalized, over time. As the first quantitatively characterized morphogenetic 
      field, this system and its behavior constitute an extraordinarily rich set of 
      materials for the study of canalization and embryonic regulation at the molecular 
      level.
FAU - Surkova, Svetlana
AU  - Surkova S
AD  - Department of Computational Biology, Center for Advanced Studies, St. Petersburg 
      State Polytechnical University, 29 Polytehnicheskaya Street, St. Petersburg, 
      195251, Russia.
FAU - Kosman, David
AU  - Kosman D
FAU - Kozlov, Konstantin
AU  - Kozlov K
FAU - Manu
AU  - Manu
FAU - Myasnikova, Ekaterina
AU  - Myasnikova E
FAU - Samsonova, Anastasia A
AU  - Samsonova AA
FAU - Spirov, Alexander
AU  - Spirov A
FAU - Vanario-Alonso, Carlos E
AU  - Vanario-Alonso CE
FAU - Samsonova, Maria
AU  - Samsonova M
FAU - Reinitz, John
AU  - Reinitz J
LA  - eng
GR  - R01 GM072022-04/GM/NIGMS NIH HHS/United States
GR  - R01 GM072022/GM/NIGMS NIH HHS/United States
GR  - RR07801/RR/NCRR NIH HHS/United States
GR  - R56 GM072022/GM/NIGMS NIH HHS/United States
GR  - R01 RR007801-17/RR/NCRR NIH HHS/United States
GR  - R01 RR007801/RR/NCRR NIH HHS/United States
GR  - GM072022/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20071104
PL  - United States
TA  - Dev Biol
JT  - Developmental biology
JID - 0372762
RN  - 0 (Drosophila Proteins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (eve protein, Drosophila)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Blastoderm/cytology/metabolism
MH  - *Body Patterning/genetics
MH  - Drosophila/*embryology/genetics/metabolism
MH  - Drosophila Proteins/metabolism
MH  - Embryonic Development
MH  - *Gene Expression
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Developmental
MH  - *Genes, Insect
MH  - Homeodomain Proteins/metabolism
MH  - *Morphogenesis
MH  - Reproducibility of Results
MH  - Time Factors
MH  - Transcription Factors/metabolism
PMC - PMC2254320
MID - NIHMS39268
EDAT- 2007/12/11 09:00
MHDA- 2008/02/27 09:00
PMCR- 2009/01/15
CRDT- 2007/12/11 09:00
PHST- 2007/04/12 00:00 [received]
PHST- 2007/08/27 00:00 [revised]
PHST- 2007/10/18 00:00 [accepted]
PHST- 2007/12/11 09:00 [pubmed]
PHST- 2008/02/27 09:00 [medline]
PHST- 2007/12/11 09:00 [entrez]
PHST- 2009/01/15 00:00 [pmc-release]
AID - S0012-1606(07)01466-2 [pii]
AID - 10.1016/j.ydbio.2007.10.037 [doi]
PST - ppublish
SO  - Dev Biol. 2008 Jan 15;313(2):844-62. doi: 10.1016/j.ydbio.2007.10.037. Epub 2007 
      Nov 4.