PMID- 18164266
OWN - NLM
STAT- MEDLINE
DCOM- 20080402
LR  - 20211203
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1783
IP  - 3
DP  - 2008 Mar
TI  - Differential roles of p80- and p130-angiomotin in the switch between migration 
      and stabilization of endothelial cells.
PG  - 429-37
LID - 10.1016/j.bbamcr.2007.11.018 [doi]
AB  - We have previously shown that angiomotin (Amot) plays an important role in growth 
      factor-induced migration of endothelial cells in vitro. Genetic knock-down of 
      Amot in zebrafish also results in inhibition of migration of intersegmental 
      vessels in vivo. Amot is expressed as two different isoforms, p80-Amot and 
      p130-Amot. Here we have analyzed the expression of the two Amot isoforms during 
      retinal angiogenesis in vivo and demonstrate that p80-Amot is expressed during 
      the migratory phase. In contrast, p130-Amot is expressed during the period of 
      blood vessel stabilization and maturation. We also show that the N-terminal 
      domain of p130-Amot serves as a targeting domain responsible for localization of 
      p130-Amot to actin and tight junctions. We further show that the relative 
      expression levels of p80-Amot and p130-Amot regulate a switch between a migratory 
      and a non-migratory cell phenotype where the migratory function of p80-Amot is 
      dominant over the stabilization and maturation function of p130-Amot. Our data 
      indicates that homo-oligomerization of p80-Amot and hetero-oligomerization of 
      both isoforms are critical for this regulation.
FAU - Ernkvist, Mira
AU  - Ernkvist M
AD  - Cancer Centrum Karolinska, Department of Oncology and Pathology, Karolinska 
      Institutet, CCK R8:03, 171 76 Stockholm, Sweden.
FAU - Birot, Olivier
AU  - Birot O
FAU - Sinha, Indranil
AU  - Sinha I
FAU - Veitonmaki, Niina
AU  - Veitonmaki N
FAU - Nystrom, Staffan
AU  - Nystrom S
FAU - Aase, Karin
AU  - Aase K
FAU - Holmgren, Lars
AU  - Holmgren L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071208
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Amot protein, mouse)
RN  - 0 (Angiomotins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Microfilament Proteins)
RN  - 0 (Protein Isoforms)
SB  - IM
MH  - Angiomotins
MH  - Animals
MH  - Animals, Newborn
MH  - CHO Cells
MH  - Cell Adhesion/*physiology
MH  - Cell Communication/physiology
MH  - Cell Movement/*physiology
MH  - Cells, Cultured
MH  - Cricetinae
MH  - Cricetulus
MH  - Dimerization
MH  - Dogs
MH  - Endothelial Cells/metabolism/*physiology
MH  - Intercellular Signaling Peptides and Proteins/metabolism/*physiology
MH  - Mice
MH  - Microfilament Proteins/metabolism/*physiology
MH  - Neovascularization, Physiologic/physiology
MH  - Protein Binding
MH  - Protein Isoforms/metabolism/physiology
MH  - Protein Transport
MH  - Retinal Vessels/growth & development/metabolism
EDAT- 2008/01/01 09:00
MHDA- 2008/04/03 09:00
CRDT- 2008/01/01 09:00
PHST- 2007/07/18 00:00 [received]
PHST- 2007/11/21 00:00 [revised]
PHST- 2007/11/26 00:00 [accepted]
PHST- 2008/01/01 09:00 [pubmed]
PHST- 2008/04/03 09:00 [medline]
PHST- 2008/01/01 09:00 [entrez]
AID - S0167-4889(07)00284-4 [pii]
AID - 10.1016/j.bbamcr.2007.11.018 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2008 Mar;1783(3):429-37. doi: 10.1016/j.bbamcr.2007.11.018. 
      Epub 2007 Dec 8.