PMID- 18171944
OWN - NLM
STAT- MEDLINE
DCOM- 20080207
LR  - 20220316
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 28
IP  - 1
DP  - 2008 Jan 2
TI  - A transcriptome database for astrocytes, neurons, and oligodendrocytes: a new 
      resource for understanding brain development and function.
PG  - 264-78
LID - 10.1523/JNEUROSCI.4178-07.2008 [doi]
AB  - Understanding the cell-cell interactions that control CNS development and 
      function has long been limited by the lack of methods to cleanly separate neural 
      cell types. Here we describe methods for the prospective isolation and 
      purification of astrocytes, neurons, and oligodendrocytes from developing and 
      mature mouse forebrain. We used FACS (fluorescent-activated cell sorting) to 
      isolate astrocytes from transgenic mice that express enhanced green fluorescent 
      protein (EGFP) under the control of an S100beta promoter. Using Affymetrix 
      GeneChip Arrays, we then created a transcriptome database of the expression 
      levels of >20,000 genes by gene profiling these three main CNS neural cell types 
      at various postnatal ages between postnatal day 1 (P1) and P30. This database 
      provides a detailed global characterization and comparison of the genes expressed 
      by acutely isolated astrocytes, neurons, and oligodendrocytes. We found that 
      Aldh1L1 is a highly specific antigenic marker for astrocytes with a substantially 
      broader pattern of astrocyte expression than the traditional astrocyte marker 
      GFAP. Astrocytes were enriched in specific metabolic and lipid synthetic 
      pathways, as well as the draper/Megf10 and Mertk/integrin alpha(v)beta5 
      phagocytic pathways suggesting that astrocytes are professional phagocytes. Our 
      findings call into question the concept of a "glial" cell class as the gene 
      profiles of astrocytes and oligodendrocytes are as dissimilar to each other as 
      they are to neurons. This transcriptome database of acutely isolated purified 
      astrocytes, neurons, and oligodendrocytes provides a resource to the neuroscience 
      community by providing improved cell-type-specific markers and for better 
      understanding of neural development, function, and disease.
FAU - Cahoy, John D
AU  - Cahoy JD
AD  - Department of Neurobiology, Stanford University School of Medicine, Stanford, 
      California 94305, USA. jcahoy@stanford.edu
FAU - Emery, Ben
AU  - Emery B
FAU - Kaushal, Amit
AU  - Kaushal A
FAU - Foo, Lynette C
AU  - Foo LC
FAU - Zamanian, Jennifer L
AU  - Zamanian JL
FAU - Christopherson, Karen S
AU  - Christopherson KS
FAU - Xing, Yi
AU  - Xing Y
FAU - Lubischer, Jane L
AU  - Lubischer JL
FAU - Krieg, Paul A
AU  - Krieg PA
FAU - Krupenko, Sergey A
AU  - Krupenko SA
FAU - Thompson, Wesley J
AU  - Thompson WJ
FAU - Barres, Ben A
AU  - Barres BA
LA  - eng
GR  - R01 NS045621/NS/NINDS NIH HHS/United States
GR  - R01 DK054388/DK/NIDDK NIH HHS/United States
GR  - F32 EY007033/EY/NEI NIH HHS/United States
GR  - R01 CA095030/CA/NCI NIH HHS/United States
GR  - R01 EY010257/EY/NEI NIH HHS/United States
GR  - T32 EY007033/EY/NEI NIH HHS/United States
GR  - CA095030/CA/NCI NIH HHS/United States
GR  - T32 GM007365/GM/NIGMS NIH HHS/United States
GR  - GM07365/GM/NIGMS NIH HHS/United States
GR  - DK54388/DK/NIDDK NIH HHS/United States
GR  - EY07033/EY/NEI NIH HHS/United States
GR  - R01 EY10257/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Astrocytes/*physiology
MH  - *Brain/cytology/growth & development/metabolism
MH  - *Gene Expression Profiling
MH  - Gene Expression Regulation, Developmental/physiology
MH  - Green Fluorescent Proteins/genetics/metabolism
MH  - Mice
MH  - Mice, Transgenic
MH  - Neurons/*physiology
MH  - Oligodendroglia/*physiology
MH  - Oligonucleotide Array Sequence Analysis/methods
MH  - *Transcription, Genetic
PMC - PMC6671143
EDAT- 2008/01/04 09:00
MHDA- 2008/02/08 09:00
PMCR- 2008/07/02
CRDT- 2008/01/04 09:00
PHST- 2008/01/04 09:00 [pubmed]
PHST- 2008/02/08 09:00 [medline]
PHST- 2008/01/04 09:00 [entrez]
PHST- 2008/07/02 00:00 [pmc-release]
AID - 28/1/264 [pii]
AID - 3301574 [pii]
AID - 10.1523/JNEUROSCI.4178-07.2008 [doi]
PST - ppublish
SO  - J Neurosci. 2008 Jan 2;28(1):264-78. doi: 10.1523/JNEUROSCI.4178-07.2008.