PMID- 18203900
OWN - NLM
STAT- MEDLINE
DCOM- 20080214
LR  - 20230216
IS  - 1541-6100 (Electronic)
IS  - 0022-3166 (Linking)
VI  - 138
IP  - 2
DP  - 2008 Feb
TI  - Dietary soy protein isolate ameliorates atherosclerotic lesions in apolipoprotein 
      E-deficient mice potentially by inhibiting monocyte chemoattractant protein-1 
      expression.
PG  - 332-7
AB  - Soy-based diets reportedly protect against the development of atherosclerosis; 
      however, the underlying mechanism(s) for this protection remains unknown. In this 
      report, the mechanism(s) contributing to the atheroprotective effects of a 
      soy-based diet was addressed using the apolipoprotein E knockout (apoE-/-) mice 
      fed soy protein isolate (SPI) associated with or without phytochemicals (SPI+ and 
      SPI-, respectively) or casein (CAS). Reduced atherosclerotic lesions were 
      observed in aortic sinus and enface analyses of the descending aorta in SPI+- or 
      SPI(-)-fed apoE-/- mice compared with CAS-fed mice. SPI+-fed mice showed 20% 
      fewer lesions compared with SPI(-)-fed mice. Plasma lipid profiles did not differ 
      among the 3 groups, suggesting alternative mechanism(s) could have contributed to 
      the atheroprotective effect of soy-based diets. Real-time quantitative PCR 
      analyses of proximal aorta showed reduced expression of monocyte chemoattractant 
      protein-1 (MCP-1), a monocyte chemokine, in mice fed both soy-based diets 
      compared with the CAS-fed mice. These findings paralleled the reduced number of 
      macrophages observed in the lesion site in the aorta of SPI+- or SPI(-)-fed mice 
      compared with CAS-fed mice. In an in vitro LPS-induced inflammation model, soy 
      isoflavones (genistein, daidzein, and equol alone or in combination) dose 
      dependently inhibited LPS-induced MCP-1 secretion by macrophages, suggesting a 
      role for soy isoflavones for the protective in vivo effects. Collectively, these 
      findings suggest that the reduction in atherosclerotic lesions observed in mice 
      fed the soy-based diet is mediated in part by inhibition of MCP-1 that could 
      result in reduced monocyte migration, an early event during atherogenesis.
FAU - Nagarajan, Shanmugam
AU  - Nagarajan S
AD  - Arkansas Children's Nutrition Center, University of Arkansas for Medical 
      Sciences, Little Rock, AR 72205, USA. nagarajanshanmugam@uams.edu
FAU - Burris, Ramona L
AU  - Burris RL
FAU - Stewart, Bradford W
AU  - Stewart BW
FAU - Wilkerson, James E
AU  - Wilkerson JE
FAU - Badger, Thomas M
AU  - Badger TM
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Nutr
JT  - The Journal of nutrition
JID - 0404243
RN  - 0 (Apolipoproteins E)
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Dietary Proteins)
RN  - 0 (Soybean Proteins)
SB  - IM
MH  - Animals
MH  - Aorta/pathology
MH  - Apolipoproteins E/*deficiency/genetics
MH  - Atherosclerosis/*pathology/*prevention & control
MH  - Cell Line
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Diet
MH  - Dietary Proteins/*pharmacology
MH  - Down-Regulation/*drug effects
MH  - Female
MH  - Humans
MH  - Macrophages/drug effects
MH  - Mice
MH  - Mice, Knockout
MH  - Soybean Proteins/*pharmacology
EDAT- 2008/01/22 09:00
MHDA- 2008/02/15 09:00
CRDT- 2008/01/22 09:00
PHST- 2008/01/22 09:00 [pubmed]
PHST- 2008/02/15 09:00 [medline]
PHST- 2008/01/22 09:00 [entrez]
AID - S0022-3166(22)09547-5 [pii]
AID - 10.1093/jn/138.2.332 [doi]
PST - ppublish
SO  - J Nutr. 2008 Feb;138(2):332-7. doi: 10.1093/jn/138.2.332.