PMID- 18218067
OWN - NLM
STAT- MEDLINE
DCOM- 20101018
LR  - 20240109
IS  - 1474-760X (Electronic)
IS  - 1474-7596 (Print)
IS  - 1474-7596 (Linking)
VI  - 9
IP  - 1
DP  - 2008 Jan 24
TI  - Novel insights into the relationships between dendritic cell subsets in human and 
      mouse revealed by genome-wide expression profiling.
PG  - R17
LID - 10.1186/gb-2008-9-1-r17 [doi]
AB  - BACKGROUND: Dendritic cells (DCs) are a complex group of cells that play a 
      critical role in vertebrate immunity. Lymph-node resident DCs (LN-DCs) are 
      subdivided into conventional DC (cDC) subsets (CD11b and CD8alpha in mouse; BDCA1 
      and BDCA3 in human) and plasmacytoid DCs (pDCs). It is currently unclear if these 
      various DC populations belong to a unique hematopoietic lineage and if the 
      subsets identified in the mouse and human systems are evolutionary homologs. To 
      gain novel insights into these questions, we sought conserved genetic signatures 
      for LN-DCs and in vitro derived granulocyte-macrophage colony stimulating factor 
      (GM-CSF) DCs through the analysis of a compendium of genome-wide expression 
      profiles of mouse or human leukocytes. RESULTS: We show through clustering 
      analysis that all LN-DC subsets form a distinct branch within the leukocyte 
      family tree, and reveal a transcriptomal signature evolutionarily conserved in 
      all LN-DC subsets. Moreover, we identify a large gene expression program shared 
      between mouse and human pDCs, and smaller conserved profiles shared between mouse 
      and human LN-cDC subsets. Importantly, most of these genes have not been 
      previously associated with DC function and many have unknown functions. Finally, 
      we use compendium analysis to re-evaluate the classification of 
      interferon-producing killer DCs, lin-CD16+HLA-DR+ cells and in vitro derived 
      GM-CSF DCs, and show that these cells are more closely linked to natural killer 
      and myeloid cells, respectively. CONCLUSION: Our study provides a unique database 
      resource for future investigation of the evolutionarily conserved molecular 
      pathways governing the ontogeny and functions of leukocyte subsets, especially 
      DCs.
FAU - Robbins, Scott H
AU  - Robbins SH
AD  - CIML (Centre d'Immunologie de Marseille-Luminy), Universite de la Mediterranee, 
      Parc scientifique de Luminy case 906, Marseille F-13288, France.
FAU - Walzer, Thierry
AU  - Walzer T
FAU - Dembele, Doulaye
AU  - Dembele D
FAU - Thibault, Christelle
AU  - Thibault C
FAU - Defays, Axel
AU  - Defays A
FAU - Bessou, Gilles
AU  - Bessou G
FAU - Xu, Huichun
AU  - Xu H
FAU - Vivier, Eric
AU  - Vivier E
FAU - Sellars, Maclean
AU  - Sellars M
FAU - Pierre, Philippe
AU  - Pierre P
FAU - Sharp, Franck R
AU  - Sharp FR
FAU - Chan, Susan
AU  - Chan S
FAU - Kastner, Philippe
AU  - Kastner P
FAU - Dalod, Marc
AU  - Dalod M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080124
PL  - England
TA  - Genome Biol
JT  - Genome biology
JID - 100960660
SB  - IM
MH  - Animals
MH  - Cell Lineage/*genetics
MH  - Cluster Analysis
MH  - Dendritic Cells/*cytology
MH  - *Gene Expression Profiling
MH  - Genome/*genetics
MH  - Genome, Human
MH  - Humans
MH  - Leukocytes
MH  - Mice
PMC - PMC2395256
EDAT- 2008/01/26 09:00
MHDA- 2010/10/19 06:00
PMCR- 2008/01/24
CRDT- 2008/01/26 09:00
PHST- 2007/08/28 00:00 [received]
PHST- 2007/12/19 00:00 [revised]
PHST- 2008/01/24 00:00 [accepted]
PHST- 2008/01/26 09:00 [pubmed]
PHST- 2010/10/19 06:00 [medline]
PHST- 2008/01/26 09:00 [entrez]
PHST- 2008/01/24 00:00 [pmc-release]
AID - gb-2008-9-1-r17 [pii]
AID - 10.1186/gb-2008-9-1-r17 [doi]
PST - epublish
SO  - Genome Biol. 2008 Jan 24;9(1):R17. doi: 10.1186/gb-2008-9-1-r17.