PMID- 18227514
OWN - NLM
STAT- MEDLINE
DCOM- 20080307
LR  - 20220318
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 105
IP  - 5
DP  - 2008 Feb 5
TI  - MicroRNA-373 induces expression of genes with complementary promoter sequences.
PG  - 1608-13
LID - 10.1073/pnas.0707594105 [doi]
AB  - Recent studies have shown that microRNA (miRNA) regulates gene expression by 
      repressing translation or directing sequence-specific degradation of 
      complementary mRNA. Here, we report new evidence in which miRNA may also function 
      to induce gene expression. By scanning gene promoters in silico for sequences 
      complementary to known miRNAs, we identified a putative miR-373 target site in 
      the promoter of E-cadherin. Transfection of miR-373 and its precursor hairpin RNA 
      (pre-miR-373) into PC-3 cells readily induced E-cadherin expression. Knockdown 
      experiments confirmed that induction of E-cadherin by pre-miR-373 required the 
      miRNA maturation protein Dicer. Further analysis revealed that cold-shock 
      domain-containing protein C2 (CSDC2), which possesses a putative miR-373 target 
      site within its promoter, was also readily induced in response to miR-373 and 
      pre-miR-373. Furthermore, enrichment of RNA polymerase II was detected at both 
      E-cadherin and CSDC2 promoters after miR-373 transfection. Mismatch mutations to 
      miR-373 indicated that gene induction was specific to the miR-373 sequence. 
      Transfection of promoter-specific dsRNAs revealed that the concurrent induction 
      of E-cadherin and CSDC2 by miR-373 required the miRNA target sites in both 
      promoters. In conclusion, we have identified a miRNA that targets promoter 
      sequences and induces gene expression. These findings reveal a new mode by which 
      miRNAs may regulate gene expression.
FAU - Place, Robert F
AU  - Place RF
AD  - Department of Urology, Veterans Affairs Medical Center and University of 
      California, San Francisco, CA 94121, USA. place.robert@gmail.com
FAU - Li, Long-Cheng
AU  - Li LC
FAU - Pookot, Deepa
AU  - Pookot D
FAU - Noonan, Emily J
AU  - Noonan EJ
FAU - Dahiya, Rajvir
AU  - Dahiya R
LA  - eng
GR  - R01CA101844/CA/NCI NIH HHS/United States
GR  - R01CA111470/CA/NCI NIH HHS/United States
GR  - T32DK007790/DK/NIDDK NIH HHS/United States
GR  - T32 DK007790/DK/NIDDK NIH HHS/United States
GR  - R01 CA101844/CA/NCI NIH HHS/United States
GR  - R01 CA111470/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20080128
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (CSDC2 protein, human)
RN  - 0 (Cadherins)
RN  - 0 (MIRN373 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA-Binding Proteins)
RN  - EC 2.7.7.- (RNA Polymerase II)
RN  - EC 3.1.26.3 (Ribonuclease III)
SB  - IM
EIN - Proc Natl Acad Sci U S A. 2018 Mar 19;:. PMID: 29555737
MH  - Base Pair Mismatch
MH  - Base Sequence
MH  - Cadherins/genetics
MH  - Cell Line, Tumor
MH  - Gene Expression/drug effects
MH  - *Gene Expression Regulation
MH  - Humans
MH  - MicroRNAs/genetics/pharmacology/*physiology
MH  - Molecular Sequence Data
MH  - Promoter Regions, Genetic/drug effects
MH  - RNA Polymerase II/metabolism
MH  - RNA-Binding Proteins/genetics
MH  - Ribonuclease III/antagonists & inhibitors/genetics/metabolism
MH  - *Transcriptional Activation
PMC - PMC2234192
COIS- The authors declare no conflict of interest.
EDAT- 2008/01/30 09:00
MHDA- 2008/03/08 09:00
PMCR- 2008/08/05
CRDT- 2008/01/30 09:00
PHST- 2008/01/30 09:00 [pubmed]
PHST- 2008/03/08 09:00 [medline]
PHST- 2008/01/30 09:00 [entrez]
PHST- 2008/08/05 00:00 [pmc-release]
AID - 0707594105 [pii]
AID - 9140 [pii]
AID - 10.1073/pnas.0707594105 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2008 Feb 5;105(5):1608-13. doi: 
      10.1073/pnas.0707594105. Epub 2008 Jan 28.