PMID- 18241851
OWN - NLM
STAT- MEDLINE
DCOM- 20080327
LR  - 20211020
IS  - 1095-564X (Electronic)
IS  - 0012-1606 (Print)
IS  - 0012-1606 (Linking)
VI  - 315
IP  - 2
DP  - 2008 Mar 15
TI  - Drosophila growth and development in the absence of dMyc and dMnt.
PG  - 303-16
LID - 10.1016/j.ydbio.2007.12.026 [doi]
AB  - Myc oncoproteins are essential regulators of the growth and proliferation of 
      mammalian cells. In Drosophila the single ortholog of Myc (dMyc), encoded by the 
      dm gene, influences organismal size and the growth of both mitotic and 
      endoreplicating cells. A null mutation in dm results in attenuated 
      endoreplication and growth arrest early in larval development. Drosophila also 
      contains a single ortholog of the mammalian Mad/Mnt transcriptional repressor 
      proteins (dMnt), which is thought to antagonize dMyc function. Here we show that 
      animals lacking both dMyc and dMnt display increased viability and grow 
      significantly larger and develop further than dMyc single mutants. We observe 
      increased endoreplication and growth of larval tissues in these double mutants 
      and disproportionate growth of the imaginal discs. Gene expression analysis 
      indicates that loss of dMyc leads to decreased expression of genes required for 
      ribosome biogenesis and protein synthesis. The additional loss of dMnt partially 
      rescues expression of a small number of dMyc and dMnt genes that are primarily 
      involved in rRNA synthesis and processing. Our results indicate that dMnt 
      repression is normally overridden by dMyc activation during larval development. 
      Therefore the severity of the dm null phenotype is likely due to unopposed 
      repression by dMnt on a subset of genes critical for cell and organismal growth. 
      Surprisingly, considerable growth and development can occur in the absence of 
      both dMyc and dMnt.
FAU - Pierce, Sarah B
AU  - Pierce SB
AD  - Division of Basic Sciences, Fred Hutchinson Cancer Research Center, MS A2-025, 
      POB 19024, Seattle, WA 98109-1024, USA.
FAU - Yost, Cynthia
AU  - Yost C
FAU - Anderson, Sarah A R
AU  - Anderson SA
FAU - Flynn, Erin M
AU  - Flynn EM
FAU - Delrow, Jeffrey
AU  - Delrow J
FAU - Eisenman, Robert N
AU  - Eisenman RN
LA  - eng
GR  - R01 CA057138/CA/NCI NIH HHS/United States
GR  - R37 CA057138/CA/NCI NIH HHS/United States
GR  - R37 CA057138-16/CA/NCI NIH HHS/United States
GR  - R37CA57138/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20071227
PL  - United States
TA  - Dev Biol
JT  - Developmental biology
JID - 0372762
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (DNA Primers)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Myc protein, Drosophila)
RN  - 0 (Repressor Proteins)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Basic Helix-Loop-Helix Transcription Factors/deficiency/genetics/physiology
MH  - DNA Primers/genetics
MH  - DNA-Binding Proteins/deficiency/*genetics/physiology
MH  - Drosophila/*genetics/*growth & development/physiology
MH  - Drosophila Proteins/deficiency/*genetics/physiology
MH  - Female
MH  - Gene Deletion
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Developmental
MH  - *Genes, Insect
MH  - Larva/growth & development
MH  - Male
MH  - Metamorphosis, Biological
MH  - *Mutation
MH  - Phenotype
MH  - Repressor Proteins/*genetics/physiology
MH  - Signal Transduction
MH  - Transcription Factors/deficiency/*genetics/physiology
MH  - Wings, Animal/growth & development
PMC - PMC2322934
MID - NIHMS43178
EDAT- 2008/02/05 09:00
MHDA- 2008/03/28 09:00
PMCR- 2009/03/15
CRDT- 2008/02/05 09:00
PHST- 2007/10/08 00:00 [received]
PHST- 2007/12/03 00:00 [revised]
PHST- 2007/12/11 00:00 [accepted]
PHST- 2008/02/05 09:00 [pubmed]
PHST- 2008/03/28 09:00 [medline]
PHST- 2008/02/05 09:00 [entrez]
PHST- 2009/03/15 00:00 [pmc-release]
AID - S0012-1606(07)01597-7 [pii]
AID - 10.1016/j.ydbio.2007.12.026 [doi]
PST - ppublish
SO  - Dev Biol. 2008 Mar 15;315(2):303-16. doi: 10.1016/j.ydbio.2007.12.026. Epub 2007 
      Dec 27.