PMID- 18536716
OWN - NLM
STAT- MEDLINE
DCOM- 20080722
LR  - 20220330
IS  - 1546-1718 (Electronic)
IS  - 1061-4036 (Print)
IS  - 1061-4036 (Linking)
VI  - 40
IP  - 7
DP  - 2008 Jul
TI  - Bmi1 is expressed in vivo in intestinal stem cells.
PG  - 915-20
LID - 10.1038/ng.165 [doi]
AB  - Bmi1 plays an essential part in the self-renewal of hematopoietic and neural stem 
      cells. To investigate its role in other adult stem cell populations, we generated 
      a mouse expressing a tamoxifen-inducible Cre from the Bmi1 locus. We found that 
      Bmi1 is expressed in discrete cells located near the bottom of crypts in the 
      small intestine, predominantly four cells above the base of the crypt (+4 
      position). Over time, these cells proliferate, expand, self-renew and give rise 
      to all the differentiated cell lineages of the small intestine epithelium. The 
      induction of a stable form of beta-catenin in these cells was sufficient to 
      rapidly generate adenomas. Moreover, ablation of Bmi1(+) cells using a Rosa26 
      conditional allele, expressing diphtheria toxin, led to crypt loss. These 
      experiments identify Bmi1 as an intestinal stem cell marker in vivo. 
      Unexpectedly, the distribution of Bmi1-expressing stem cells along the length of 
      the small intestine suggested that mammals use more than one molecularly 
      distinguishable adult stem cell subpopulation to maintain organ homeostasis.
FAU - Sangiorgi, Eugenio
AU  - Sangiorgi E
AD  - Howard Hughes Medical Institute and Department of Human Genetics, University of 
      Utah School of Medicine, Salt Lake City, Utah 84112, USA.
FAU - Capecchi, Mario R
AU  - Capecchi MR
LA  - eng
GR  - R01 GM021168/GM/NIGMS NIH HHS/United States
GR  - R01 GM021168-34/GM/NIGMS NIH HHS/United States
GR  - HHMI/Howard Hughes Medical Institute/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080608
PL  - United States
TA  - Nat Genet
JT  - Nature genetics
JID - 9216904
RN  - 0 (Bmi1 protein, mouse)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Repressor Proteins)
RN  - EC 2.3.2.27 (Polycomb Repressive Complex 1)
SB  - IM
CIN - Nat Genet. 2008 Jul;40(7):818-9. doi: 10.1038/ng0708-818. PMID: 18583974
MH  - Adult Stem Cells/*metabolism
MH  - Animals
MH  - Homeostasis/physiology
MH  - Intestinal Mucosa/*metabolism
MH  - Intestines/cytology/physiology
MH  - Lac Operon
MH  - Mice
MH  - Mice, Transgenic
MH  - Nuclear Proteins/genetics/*metabolism
MH  - Polycomb Repressive Complex 1
MH  - Proto-Oncogene Proteins/genetics/*metabolism
MH  - Repressor Proteins/genetics/*metabolism
MH  - Tissue Distribution
PMC - PMC2906135
MID - NIHMS128707
EDAT- 2008/06/10 09:00
MHDA- 2008/07/23 09:00
PMCR- 2010/07/19
CRDT- 2008/06/10 09:00
PHST- 2008/02/12 00:00 [received]
PHST- 2008/04/02 00:00 [accepted]
PHST- 2008/06/10 09:00 [pubmed]
PHST- 2008/07/23 09:00 [medline]
PHST- 2008/06/10 09:00 [entrez]
PHST- 2010/07/19 00:00 [pmc-release]
AID - ng.165 [pii]
AID - 10.1038/ng.165 [doi]
PST - ppublish
SO  - Nat Genet. 2008 Jul;40(7):915-20. doi: 10.1038/ng.165. Epub 2008 Jun 8.