PMID- 18546601
OWN - NLM
STAT- MEDLINE
DCOM- 20080903
LR  - 20240726
IS  - 1750-2799 (Electronic)
IS  - 1750-2799 (Linking)
VI  - 3
IP  - 6
DP  - 2008
TI  - Analyzing real-time PCR data by the comparative C(T) method.
PG  - 1101-8
AB  - Two different methods of presenting quantitative gene expression exist: absolute 
      and relative quantification. Absolute quantification calculates the copy number 
      of the gene usually by relating the PCR signal to a standard curve. Relative gene 
      expression presents the data of the gene of interest relative to some calibrator 
      or internal control gene. A widely used method to present relative gene 
      expression is the comparative C(T) method also referred to as the 2 
      (-DeltaDeltaC(T)) method. This protocol provides an overview of the comparative 
      C(T) method for quantitative gene expression studies. Also presented here are 
      various examples to present quantitative gene expression data using this method.
FAU - Schmittgen, Thomas D
AU  - Schmittgen TD
AD  - Division of Pharmaceutics, College of Pharmacy, Ohio State University, Parks 
      Hall, 500 West 12th Avenue, Columbus, Ohio, OH 43210 USA. schmittgen.2@osu.edu
FAU - Livak, Kenneth J
AU  - Livak KJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Nat Protoc
JT  - Nature protocols
JID - 101284307
RN  - 0 (Homeodomain Proteins)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factors)
RN  - 145420-66-2 (HOXD10 protein, human)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Animals
MH  - Breast Neoplasms/genetics
MH  - Computer Systems/statistics & numerical data
MH  - Data Interpretation, Statistical
MH  - Female
MH  - Gene Expression
MH  - Gene Expression Profiling/statistics & numerical data
MH  - Genes, bcl-2
MH  - Homeodomain Proteins/genetics
MH  - Humans
MH  - Mice
MH  - MicroRNAs/genetics
MH  - Pancreas/metabolism
MH  - Pancreatic Neoplasms/genetics
MH  - Polymerase Chain Reaction/methods/*statistics & numerical data
MH  - RNA/genetics
MH  - RNA, Messenger/genetics
MH  - Transcription Factors/genetics
EDAT- 2008/06/13 09:00
MHDA- 2008/09/04 09:00
CRDT- 2008/06/13 09:00
PHST- 2008/06/13 09:00 [pubmed]
PHST- 2008/09/04 09:00 [medline]
PHST- 2008/06/13 09:00 [entrez]
AID - 10.1038/nprot.2008.73 [doi]
PST - ppublish
SO  - Nat Protoc. 2008;3(6):1101-8. doi: 10.1038/nprot.2008.73.