PMID- 18805090
OWN - NLM
STAT- MEDLINE
DCOM- 20081009
LR  - 20220330
IS  - 1097-4172 (Electronic)
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 134
IP  - 6
DP  - 2008 Sep 19
TI  - Mechanism of replication-coupled DNA interstrand crosslink repair.
PG  - 969-80
LID - 10.1016/j.cell.2008.08.030 [doi]
AB  - DNA interstrand crosslinks (ICLs) are toxic DNA lesions whose repair occurs in 
      the S phase of metazoans via an unknown mechanism. Here, we describe a cell-free 
      system based on Xenopus egg extracts that supports ICL repair. During DNA 
      replication of a plasmid containing a site-specific ICL, two replication forks 
      converge on the crosslink. Subsequent lesion bypass involves advance of a nascent 
      leading strand to within one nucleotide of the ICL, followed by incisions, 
      translesion DNA synthesis, and extension of the nascent strand beyond the lesion. 
      Immunodepletion experiments suggest that extension requires DNA polymerase zeta. 
      Ultimately, a significant portion of the input DNA is fully repaired, but not if 
      DNA replication is blocked. Our experiments establish a mechanism for ICL repair 
      that reveals how this process is coupled to DNA replication.
FAU - Raschle, Markus
AU  - Raschle M
AD  - Harvard Medical School, Department of Biological Chemistry and Molecular 
      Pharmacology, Boston, MA 02115, USA.
FAU - Knipscheer, Puck
AU  - Knipscheer P
FAU - Enoiu, Milica
AU  - Enoiu M
FAU - Angelov, Todor
AU  - Angelov T
FAU - Sun, Jingchuan
AU  - Sun J
FAU - Griffith, Jack D
AU  - Griffith JD
FAU - Ellenberger, Tom E
AU  - Ellenberger TE
FAU - Scharer, Orlando D
AU  - Scharer OD
FAU - Walter, Johannes C
AU  - Walter JC
LA  - eng
GR  - GM62267/GM/NIGMS NIH HHS/United States
GR  - R01 GM062267-07/GM/NIGMS NIH HHS/United States
GR  - R01 GM062267-08/GM/NIGMS NIH HHS/United States
GR  - R01 GM062267/GM/NIGMS NIH HHS/United States
GR  - GM55390/GM/NIGMS NIH HHS/United States
GR  - GM31819/GM/NIGMS NIH HHS/United States
GR  - R01 GM031819/GM/NIGMS NIH HHS/United States
GR  - R01 GM055390/GM/NIGMS NIH HHS/United States
GR  - R01 GM062267-06/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 9007-49-2 (DNA)
RN  - EC 2.7.7.- (DNA polymerase zeta)
RN  - EC 2.7.7.7 (DNA-Directed DNA Polymerase)
SB  - IM
EIN - Cell.2009 May 29;137(5):972. Knipsheer, Puck [corrected to Knipscheer, Puck]
MH  - Animals
MH  - Cell-Free System
MH  - DNA
MH  - *DNA Repair
MH  - *DNA Replication
MH  - DNA-Directed DNA Polymerase/genetics/metabolism
MH  - Plasmids/genetics/metabolism
MH  - Xenopus
PMC - PMC2748255
MID - NIHMS70634
EDAT- 2008/09/23 09:00
MHDA- 2008/10/10 09:00
PMCR- 2009/09/22
CRDT- 2008/09/23 09:00
PHST- 2007/12/18 00:00 [received]
PHST- 2008/07/07 00:00 [revised]
PHST- 2008/08/14 00:00 [accepted]
PHST- 2008/09/23 09:00 [pubmed]
PHST- 2008/10/10 09:00 [medline]
PHST- 2008/09/23 09:00 [entrez]
PHST- 2009/09/22 00:00 [pmc-release]
AID - S0092-8674(08)01075-1 [pii]
AID - 10.1016/j.cell.2008.08.030 [doi]
PST - ppublish
SO  - Cell. 2008 Sep 19;134(6):969-80. doi: 10.1016/j.cell.2008.08.030.