PMID- 19228882
OWN - NLM
STAT- MEDLINE
DCOM- 20090602
LR  - 20220317
IS  - 0193-1857 (Print)
IS  - 1522-1547 (Electronic)
IS  - 0193-1857 (Linking)
VI  - 296
IP  - 5
DP  - 2009 May
TI  - Distinct SOX9 levels differentially mark stem/progenitor populations and 
      enteroendocrine cells of the small intestine epithelium.
PG  - G1108-18
LID - 10.1152/ajpgi.00004.2009 [doi]
AB  - SOX transcription factors have the capacity to modulate stem/progenitor cell 
      proliferation and differentiation in a dose-dependent manner. SOX9 is expressed 
      in the small intestine epithelial stem cell zone. Therefore, we hypothesized that 
      differential levels of SOX9 may exist, influencing proliferation and/or 
      differentiation of the small intestine epithelium. Sox9 expression levels in the 
      small intestine were investigated using a Sox9 enhanced green fluorescent protein 
      (Sox9(EGFP)) transgenic mouse. Sox9(EGFP) levels correlate with endogenous SOX9 
      levels, which are expressed at two steady-state levels, termed Sox9(EGFPLO) and 
      Sox9(EGFPHI). Crypt-based columnar cells are Sox9(EGFPLO) and demonstrate 
      enriched expression of the stem cell marker, Lgr5. Sox9(EGFPHI) cells express 
      chromogranin A and substance P but do not express Ki67 and neurogenin3, 
      indicating that Sox9(EGFPHI) cells are postmitotic enteroendocrine cells. 
      Overexpression of SOX9 in a crypt cell line stopped proliferation and induced 
      morphological changes. These data support a bimodal role for SOX9 in the 
      intestinal epithelium, where low SOX9 expression supports proliferative capacity, 
      and high SOX9 expression suppresses proliferation.
FAU - Formeister, Eric J
AU  - Formeister EJ
AD  - Department of Medicine, Division of Gastroenterology and Hepatology, University 
      of North Carolina at Chapel Hill, NC 27599, USA.
FAU - Sionas, Ayn L
AU  - Sionas AL
FAU - Lorance, David K
AU  - Lorance DK
FAU - Barkley, Carey L
AU  - Barkley CL
FAU - Lee, Ginny H
AU  - Lee GH
FAU - Magness, Scott T
AU  - Magness ST
LA  - eng
GR  - P30 DK034987/DK/NIDDK NIH HHS/United States
GR  - P30 NS045892/NS/NINDS NIH HHS/United States
GR  - 5P30DK034987/DK/NIDDK NIH HHS/United States
GR  - 1-K01-DK080181-01/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20090219
PL  - United States
TA  - Am J Physiol Gastrointest Liver Physiol
JT  - American journal of physiology. Gastrointestinal and liver physiology
JID - 100901227
RN  - 0 (Chromogranin A)
RN  - 0 (Lgr5 protein, mouse)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (SOX9 Transcription Factor)
RN  - 0 (Sox9 protein, mouse)
RN  - 33507-63-0 (Substance P)
SB  - IM
MH  - Animals
MH  - *Cell Differentiation/genetics
MH  - Cell Line
MH  - *Cell Proliferation
MH  - Cell Shape
MH  - Chromogranin A/metabolism
MH  - Enteroendocrine Cells/*metabolism
MH  - Gene Expression Regulation
MH  - Genes, Reporter
MH  - Genotype
MH  - Intestinal Mucosa/cytology/*metabolism
MH  - Intestine, Small/cytology/*metabolism
MH  - Mice
MH  - Mice, Transgenic
MH  - Paneth Cells/metabolism
MH  - Phenotype
MH  - Rats
MH  - Receptors, G-Protein-Coupled/metabolism
MH  - SOX9 Transcription Factor/genetics/*metabolism
MH  - Stem Cells/*metabolism
MH  - Substance P/metabolism
MH  - Transduction, Genetic
PMC - PMC2696217
EDAT- 2009/02/21 09:00
MHDA- 2009/06/03 09:00
PMCR- 2010/05/01
CRDT- 2009/02/21 09:00
PHST- 2009/02/21 09:00 [entrez]
PHST- 2009/02/21 09:00 [pubmed]
PHST- 2009/06/03 09:00 [medline]
PHST- 2010/05/01 00:00 [pmc-release]
AID - 00004.2009 [pii]
AID - GI-00004-2009 [pii]
AID - 10.1152/ajpgi.00004.2009 [doi]
PST - ppublish
SO  - Am J Physiol Gastrointest Liver Physiol. 2009 May;296(5):G1108-18. doi: 
      10.1152/ajpgi.00004.2009. Epub 2009 Feb 19.