PMID- 19269367
OWN - NLM
STAT- MEDLINE
DCOM- 20090327
LR  - 20220316
IS  - 1097-4172 (Electronic)
IS  - 0092-8674 (Linking)
VI  - 136
IP  - 5
DP  - 2009 Mar 6
TI  - Transcription factor achaete scute-like 2 controls intestinal stem cell fate.
PG  - 903-12
LID - 10.1016/j.cell.2009.01.031 [doi]
AB  - The small intestinal epithelium is the most rapidly self-renewing tissue of 
      mammals. Proliferative cells are confined to crypts, while differentiated cell 
      types predominantly occupy the villi. We recently demonstrated the existence of a 
      long-lived pool of cycling stem cells defined by Lgr5 expression and intermingled 
      with post-mitotic Paneth cells at crypt bottoms. We have now determined a gene 
      signature for these Lgr5 stem cells. One of the genes within this stem cell 
      signature is the Wnt target Achaete scute-like 2 (Ascl2). Transgenic expression 
      of the Ascl2 transcription factor throughout the intestinal epithelium induces 
      crypt hyperplasia and ectopic crypts on villi. Induced deletion of the Ascl2 gene 
      in adult small intestine leads to disappearance of the Lgr5 stem cells within 
      days. The combined results from these gain- and loss-of-function experiments 
      imply that Ascl2 controls intestinal stem cell fate.
FAU - van der Flier, Laurens G
AU  - van der Flier LG
AD  - Hubrecht Institute-KNAW & University Medical Center Utrecht, Uppsalaan, The 
      Netherlands.
FAU - van Gijn, Marielle E
AU  - van Gijn ME
FAU - Hatzis, Pantelis
AU  - Hatzis P
FAU - Kujala, Pekka
AU  - Kujala P
FAU - Haegebarth, Andrea
AU  - Haegebarth A
FAU - Stange, Daniel E
AU  - Stange DE
FAU - Begthel, Harry
AU  - Begthel H
FAU - van den Born, Maaike
AU  - van den Born M
FAU - Guryev, Victor
AU  - Guryev V
FAU - Oving, Irma
AU  - Oving I
FAU - van Es, Johan H
AU  - van Es JH
FAU - Barker, Nick
AU  - Barker N
FAU - Peters, Peter J
AU  - Peters PJ
FAU - van de Wetering, Marc
AU  - van de Wetering M
FAU - Clevers, Hans
AU  - Clevers H
LA  - eng
SI  - GEO/GSE14201
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (Ascl2 protein, mouse)
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
SB  - IM
MH  - Adult Stem Cells/*metabolism
MH  - Animals
MH  - Basic Helix-Loop-Helix Transcription Factors/*metabolism
MH  - Cell Separation
MH  - Gene Deletion
MH  - Gene Expression Profiling
MH  - Intestine, Small/*cytology
MH  - Mice
MH  - Mice, Transgenic
EDAT- 2009/03/10 09:00
MHDA- 2009/03/28 09:00
CRDT- 2009/03/10 09:00
PHST- 2008/07/29 00:00 [received]
PHST- 2008/11/07 00:00 [revised]
PHST- 2009/01/07 00:00 [accepted]
PHST- 2009/03/10 09:00 [entrez]
PHST- 2009/03/10 09:00 [pubmed]
PHST- 2009/03/28 09:00 [medline]
AID - S0092-8674(09)00079-8 [pii]
AID - 10.1016/j.cell.2009.01.031 [doi]
PST - ppublish
SO  - Cell. 2009 Mar 6;136(5):903-12. doi: 10.1016/j.cell.2009.01.031.