PMID- 19324043
OWN - NLM
STAT- MEDLINE
DCOM- 20090708
LR  - 20220316
IS  - 1528-0012 (Electronic)
IS  - 0016-5085 (Linking)
VI  - 136
IP  - 7
DP  - 2009 Jun
TI  - Prominin-1/CD133 marks stem cells and early progenitors in mouse small intestine.
PG  - 2187-2194.e1
LID - 10.1053/j.gastro.2009.03.002 [doi]
AB  - BACKGROUND & AIMS: Prominin-1(Prom1)/CD133 is used, alone or in combination with 
      other cell surface markers, to identify and isolate stem cells from various adult 
      tissues. We recently identified leucine-rich-repeat-containing G-protein-coupled 
      receptor 5 (Lgr5) as a marker of the intestinal stem cells from which all 
      cellular lineages of the gastrointestinal epithelium are derived. To determine 
      whether there is a relationship between these markers, we investigated the 
      intestinal expression pattern of Prom1/CD133 and created knock-in mice to 
      visualize and trace Prom1(+) cells. METHODS: We analyzed Prom1 mRNA and protein 
      expression among stem cells within intestinal crypts. Prom1/CD133 knock-in mice 
      (Prom1(-mCherry-IRES-CreERT2) KI) were generated that express a fusion of red 
      fluorescent protein mCherry with the C-terminus of Prom1. The knock-in allele 
      also contains the tamoxifen-inducible CreERT2 recombinase, allowing for genetic 
      tracing of progeny derived from Prom1-positive cells. RESULTS: In the small 
      intestine, Prom1 mRNA was detected throughout the lower half of crypts and was 
      not restricted to the rare stem cells that are sandwiched between Paneth cells. 
      Prom1 protein was detected at the apical membranes of Lgr5(+) intestinal stem 
      cells, but also on the transit-amplifying progenitors located above the Paneth 
      cells. Analyses of the Prom1(-mCherry-IRES-CreERT2) KI mice showed that Prom1 is 
      not exclusively expressed in Lgr5(+) intestinal stem cells but marks a much 
      larger stem cell/transit-amplifying progenitor compartment. CONCLUSIONS: Prom-1 
      marks intestinal stem cells, as well as transit-amplifying progenitors, so it is 
      not a specific marker for Lgr5(+) intestinal stem cells.
FAU - Snippert, Hugo J
AU  - Snippert HJ
AD  - Hubrecht Institute, KNAW and University Medical Center Utrecht, Utrecht, The 
      Netherlands.
FAU - van Es, Johan H
AU  - van Es JH
FAU - van den Born, Maaike
AU  - van den Born M
FAU - Begthel, Harry
AU  - Begthel H
FAU - Stange, Daniel E
AU  - Stange DE
FAU - Barker, Nick
AU  - Barker N
FAU - Clevers, Hans
AU  - Clevers H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090324
PL  - United States
TA  - Gastroenterology
JT  - Gastroenterology
JID - 0374630
RN  - 0 (AC133 Antigen)
RN  - 0 (Antigens, CD)
RN  - 0 (Genetic Markers)
RN  - 0 (Glycoproteins)
RN  - 0 (Peptides)
RN  - 0 (Prom1 protein, mouse)
RN  - 0 (RNA, Messenger)
SB  - IM
CIN - Gastroenterology. 2009 Jun;136(7):2051-4. doi: 10.1053/j.gastro.2009.04.021. 
      PMID: 19409288
MH  - AC133 Antigen
MH  - Animals
MH  - Antigens, CD/*genetics/metabolism
MH  - Cell Lineage/genetics
MH  - *Genetic Markers
MH  - Glycoproteins/*genetics/metabolism
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Intestine, Small/*cytology/pathology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Models, Animal
MH  - Peptides/*genetics/metabolism
MH  - RNA, Messenger/analysis/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sensitivity and Specificity
MH  - Stem Cells/cytology/pathology
EDAT- 2009/03/28 09:00
MHDA- 2009/07/09 09:00
CRDT- 2009/03/28 09:00
PHST- 2009/01/23 00:00 [received]
PHST- 2009/02/17 00:00 [revised]
PHST- 2009/03/04 00:00 [accepted]
PHST- 2009/03/28 09:00 [entrez]
PHST- 2009/03/28 09:00 [pubmed]
PHST- 2009/07/09 09:00 [medline]
AID - S0016-5085(09)00371-0 [pii]
AID - 10.1053/j.gastro.2009.03.002 [doi]
PST - ppublish
SO  - Gastroenterology. 2009 Jun;136(7):2187-2194.e1. doi: 
      10.1053/j.gastro.2009.03.002. Epub 2009 Mar 24.