PMID- 19329995
OWN - NLM
STAT- MEDLINE
DCOM- 20090630
LR  - 20240104
IS  - 1476-4687 (Electronic)
IS  - 0028-0836 (Linking)
VI  - 459
IP  - 7244
DP  - 2009 May 14
TI  - Single Lgr5 stem cells build crypt-villus structures in vitro without a 
      mesenchymal niche.
PG  - 262-5
LID - 10.1038/nature07935 [doi]
AB  - The intestinal epithelium is the most rapidly self-renewing tissue in adult 
      mammals. We have recently demonstrated the presence of about six cycling Lgr5(+) 
      stem cells at the bottoms of small-intestinal crypts. Here we describe the 
      establishment of long-term culture conditions under which single crypts undergo 
      multiple crypt fission events, while simultanously generating villus-like 
      epithelial domains in which all differentiated cell types are present. Single 
      sorted Lgr5(+) stem cells can also initiate these cryptvillus organoids. Tracing 
      experiments indicate that the Lgr5(+) stem-cell hierarchy is maintained in 
      organoids. We conclude that intestinal cryptvillus units are self-organizing 
      structures, which can be built from a single stem cell in the absence of a 
      non-epithelial cellular niche.
FAU - Sato, Toshiro
AU  - Sato T
AD  - Hubrecht Institute and University Medical Center Utrecht, Uppsalalaan 8, 3584CT 
      Utrecht, The Netherlands.
FAU - Vries, Robert G
AU  - Vries RG
FAU - Snippert, Hugo J
AU  - Snippert HJ
FAU - van de Wetering, Marc
AU  - van de Wetering M
FAU - Barker, Nick
AU  - Barker N
FAU - Stange, Daniel E
AU  - Stange DE
FAU - van Es, Johan H
AU  - van Es JH
FAU - Abo, Arie
AU  - Abo A
FAU - Kujala, Pekka
AU  - Kujala P
FAU - Peters, Peter J
AU  - Peters PJ
FAU - Clevers, Hans
AU  - Clevers H
LA  - eng
PT  - Journal Article
DEP - 20090329
PL  - England
TA  - Nature
JT  - Nature
JID - 0410462
RN  - 0 (Lgr5 protein, mouse)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (Receptors, Notch)
SB  - IM
MH  - Animals
MH  - Cell Culture Techniques/*methods
MH  - Cell Lineage
MH  - Cell Separation
MH  - Gene Expression Regulation, Developmental
MH  - Intestinal Mucosa/metabolism
MH  - Intestines/*anatomy & histology/*cytology
MH  - Mesoderm/cytology/metabolism
MH  - Mice
MH  - Multipotent Stem Cells/cytology/metabolism
MH  - Organoids/*cytology/growth & development/metabolism
MH  - Paneth Cells/metabolism
MH  - Receptors, G-Protein-Coupled/*metabolism
MH  - Receptors, Notch/metabolism
MH  - Regeneration
MH  - Stem Cell Niche
MH  - Stem Cells/*cytology/*metabolism
EDAT- 2009/03/31 09:00
MHDA- 2009/07/01 09:00
CRDT- 2009/03/31 09:00
PHST- 2008/07/16 00:00 [received]
PHST- 2009/02/24 00:00 [accepted]
PHST- 2009/03/31 09:00 [entrez]
PHST- 2009/03/31 09:00 [pubmed]
PHST- 2009/07/01 09:00 [medline]
AID - nature07935 [pii]
AID - 10.1038/nature07935 [doi]
PST - ppublish
SO  - Nature. 2009 May 14;459(7244):262-5. doi: 10.1038/nature07935. Epub 2009 Mar 29.