PMID- 19748581
OWN - NLM
STAT- MEDLINE
DCOM- 20091224
LR  - 20220310
IS  - 1873-2518 (Electronic)
IS  - 0264-410X (Linking)
VI  - 27
IP  - 48
DP  - 2009 Nov 12
TI  - Recombinant outer membrane vesicles to augment antigen-specific live vaccine 
      responses.
PG  - 6748-54
LID - 10.1016/j.vaccine.2009.08.106 [doi]
AB  - Release of outer membrane vesicles is a common feature of Gram negative bacteria. 
      There is growing interest in the use of these vesicles in the development of 
      affordable vaccines. However, to exploit their full potential a convenient system 
      to generate recombinant vesicles would be highly desirable. Here, we report the 
      design of a versatile system for preparation of recombinant outer membrane 
      vesicles based on an engineered autotransporter. Two model vaccine antigens of 
      Leishmania were expressed as fusion proteins with the transporter domain of AIDA, 
      the Escherichia coli adhesin involved in diffuse adhesion. Single subcutaneous 
      injections of recombinant vesicles boosted vaccine antigen-specific antibody 
      responses in mice primed with live recombinant salmonella vaccines by 6-40-fold. 
      The results further show an expansion of only the vaccine antigen-specific 
      antibody response indicating a great potential of this approach for prime boost 
      vaccination strategies.
FAU - Schroeder, Juliane
AU  - Schroeder J
AD  - Institute of Immunology and Infection Research, University of Edinburgh, UK.
FAU - Aebischer, Toni
AU  - Aebischer T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090911
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (AIDA-I protein, E coli)
RN  - 0 (Adhesins, Escherichia coli)
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Antibodies, Protozoan)
RN  - 0 (Bacterial Outer Membrane Proteins)
RN  - 0 (Leishmaniasis Vaccines)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Protozoan Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Vaccines, Synthetic)
RN  - 0 (kinetoplastid membrane protein 11, Leishmania donovani)
SB  - IM
MH  - Adhesins, Escherichia coli/immunology
MH  - Animals
MH  - Antibodies, Bacterial/blood
MH  - Antibodies, Protozoan/blood
MH  - *Antibody Specificity
MH  - Bacterial Outer Membrane Proteins/immunology
MH  - Female
MH  - Immunity, Humoral
MH  - Leishmania/immunology
MH  - Leishmaniasis Vaccines/*immunology
MH  - Membrane Glycoproteins/*immunology
MH  - Mice
MH  - Plasmids
MH  - Protozoan Proteins/*immunology
MH  - Recombinant Fusion Proteins/immunology
MH  - Salmonella typhimurium/immunology
MH  - Transport Vesicles/*immunology
MH  - Vaccines, Synthetic/immunology
EDAT- 2009/09/15 06:00
MHDA- 2009/12/25 06:00
CRDT- 2009/09/15 06:00
PHST- 2009/06/12 00:00 [received]
PHST- 2009/08/13 00:00 [revised]
PHST- 2009/08/22 00:00 [accepted]
PHST- 2009/09/15 06:00 [entrez]
PHST- 2009/09/15 06:00 [pubmed]
PHST- 2009/12/25 06:00 [medline]
AID - S0264-410X(09)01277-8 [pii]
AID - 10.1016/j.vaccine.2009.08.106 [doi]
PST - ppublish
SO  - Vaccine. 2009 Nov 12;27(48):6748-54. doi: 10.1016/j.vaccine.2009.08.106. Epub 
      2009 Sep 11.