PMID- 19783037
OWN - NLM
STAT- MEDLINE
DCOM- 20100125
LR  - 20220317
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Linking)
VI  - 31
IP  - 1
DP  - 2010 Jan
TI  - Self-assembled hyaluronic acid nanoparticles for active tumor targeting.
PG  - 106-14
LID - 10.1016/j.biomaterials.2009.09.030 [doi]
AB  - Hyaluronic acid nanoparticles (HA-NPs), which are formed by the self-assembly of 
      hydrophobically modified HA derivatives, were prepared to investigate their 
      physicochemical characteristics and fates in tumor-bearing mice after systemic 
      administration. The particle sizes of HA-NPs were controlled in the range of 
      237-424 nm by varying the degree of substitution of the hydrophobic moiety. When 
      SCC7 cancer cells over-expressing CD44 (the receptor for HA) were treated with 
      fluorescently labeled Cy5.5-HA-NPs, strong fluorescence signals were observed in 
      the cytosol of these cells, suggesting efficient intracellular uptake of HA-NPs 
      by receptor-mediated endocytosis. In contrast, no significant fluorescence 
      signals were observed when Cy5.5-labeled HA-NPs were incubated with normal 
      fibroblast cells (CV-1) or with excess free-HA treated SCC7 cells. Following 
      systemic administration of Cy5.5-labeled HA-NPs with different particle sizes 
      into a tumor-bearing mouse, their biodistribution was monitored as a function of 
      time using a non-invasive near-infrared fluorescence imaging system. Irrespective 
      of the particle size, significant amounts of HA-NPs circulated for two days in 
      the bloodstream and were selectively accumulated into the tumor site. The smaller 
      HA-NPs were able to reach the tumor site more effectively than larger HA-NPs. 
      Interestingly, the concentration of HA-NPs in the tumor site was dramatically 
      reduced when mice were pretreated with an excess of free-HA. These results imply 
      that HA-NPs can accumulate into the tumor site by a combination of passive and 
      active targeting mechanisms.
FAU - Choi, Ki Young
AU  - Choi KY
AD  - Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, Seoul 
      130-701, Republic of Korea.
FAU - Chung, Hyunjin
AU  - Chung H
FAU - Min, Kyung Hyun
AU  - Min KH
FAU - Yoon, Hong Yeol
AU  - Yoon HY
FAU - Kim, Kwangmeyung
AU  - Kim K
FAU - Park, Jae Hyung
AU  - Park JH
FAU - Kwon, Ick Chan
AU  - Kwon IC
FAU - Jeong, Seo Young
AU  - Jeong SY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090926
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 9004-61-9 (Hyaluronic Acid)
SB  - IM
MH  - Animals
MH  - *Drug Delivery Systems
MH  - Hyaluronic Acid/*administration & dosage
MH  - Mice
MH  - Microscopy, Confocal
MH  - *Nanoparticles
MH  - Neoplasms, Experimental/*drug therapy
EDAT- 2009/09/29 06:00
MHDA- 2010/01/26 06:00
CRDT- 2009/09/29 06:00
PHST- 2009/08/18 00:00 [received]
PHST- 2009/09/08 00:00 [accepted]
PHST- 2009/09/29 06:00 [entrez]
PHST- 2009/09/29 06:00 [pubmed]
PHST- 2010/01/26 06:00 [medline]
AID - S0142-9612(09)00960-0 [pii]
AID - 10.1016/j.biomaterials.2009.09.030 [doi]
PST - ppublish
SO  - Biomaterials. 2010 Jan;31(1):106-14. doi: 10.1016/j.biomaterials.2009.09.030. 
      Epub 2009 Sep 26.