PMID- 19797770
OWN - NLM
STAT- MEDLINE
DCOM- 20091014
LR  - 20211020
IS  - 1549-5477 (Electronic)
IS  - 0890-9369 (Print)
IS  - 0890-9369 (Linking)
VI  - 23
IP  - 19
DP  - 2009 Oct 1
TI  - Invasive and indigenous microbiota impact intestinal stem cell activity through 
      multiple pathways in Drosophila.
PG  - 2333-44
LID - 10.1101/gad.1827009 [doi]
AB  - Gut homeostasis is controlled by both immune and developmental mechanisms, and 
      its disruption can lead to inflammatory disorders or cancerous lesions of the 
      intestine. While the impact of bacteria on the mucosal immune system is beginning 
      to be precisely understood, little is known about the effects of bacteria on gut 
      epithelium renewal. Here, we addressed how both infectious and indigenous 
      bacteria modulate stem cell activity in Drosophila. We show that the increased 
      epithelium renewal observed upon some bacterial infections is a consequence of 
      the oxidative burst, a major defense of the Drosophila gut. Additionally, we 
      provide evidence that the JAK-STAT (Janus kinase-signal transducers and 
      activators of transcription) and JNK (c-Jun NH(2) terminal kinase) pathways are 
      both required for bacteria-induced stem cell proliferation. Similarly, we 
      demonstrate that indigenous gut microbiota activate the same, albeit reduced, 
      program at basal levels. Altered control of gut microbiota in immune-deficient or 
      aged flies correlates with increased epithelium renewal. Finally, we show that 
      epithelium renewal is an essential component of Drosophila defense against oral 
      bacterial infection. Altogether, these results indicate that gut homeostasis is 
      achieved by a complex interregulation of the immune response, gut microbiota, and 
      stem cell activity.
FAU - Buchon, Nicolas
AU  - Buchon N
AD  - Global Health Institute, Ecole Polytechnique Federale de Lausanne (EPFL), CH-1015 
      Lausanne, Switzerland. nicolas.buchon@epfl.ch
FAU - Broderick, Nichole A
AU  - Broderick NA
FAU - Chakrabarti, Sveta
AU  - Chakrabarti S
FAU - Lemaitre, Bruno
AU  - Lemaitre B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Dev
JT  - Genes & development
JID - 8711660
RN  - 0 (Drosophila Proteins)
RN  - 0 (STAT Transcription Factors)
RN  - 0 (Transcription Factors)
RN  - EC 2.7.10.2 (Janus Kinases)
RN  - EC 2.7.10.2 (hop protein, Drosophila)
SB  - IM
CIN - Genes Dev. 2009 Oct 1;23(19):2260-5. PMID: 19797765
MH  - Animals
MH  - Cell Proliferation
MH  - Drosophila Proteins/metabolism
MH  - Drosophila melanogaster/*cytology/*microbiology
MH  - Epithelium/microbiology
MH  - Gene Expression Regulation, Developmental
MH  - Intestines/cytology/immunology/microbiology
MH  - Janus Kinases/metabolism
MH  - Pectobacterium carotovorum/*physiology
MH  - Pseudomonas/*physiology
MH  - Respiratory Burst
MH  - STAT Transcription Factors/metabolism
MH  - Stem Cells/cytology/microbiology
MH  - Transcription Factors/metabolism
PMC - PMC2758745
EDAT- 2009/10/03 06:00
MHDA- 2009/10/15 06:00
PMCR- 2010/04/01
CRDT- 2009/10/03 06:00
PHST- 2009/10/03 06:00 [entrez]
PHST- 2009/10/03 06:00 [pubmed]
PHST- 2009/10/15 06:00 [medline]
PHST- 2010/04/01 00:00 [pmc-release]
AID - 23/19/2333 [pii]
AID - 10.1101/gad.1827009 [doi]
PST - ppublish
SO  - Genes Dev. 2009 Oct 1;23(19):2333-44. doi: 10.1101/gad.1827009.