PMID- 19853561
OWN - NLM
STAT- MEDLINE
DCOM- 20091110
LR  - 20211020
IS  - 1878-1551 (Electronic)
IS  - 1534-5807 (Print)
IS  - 1534-5807 (Linking)
VI  - 17
IP  - 4
DP  - 2009 Oct
TI  - The core protein of glypican Dally-like determines its biphasic activity in 
      wingless morphogen signaling.
PG  - 470-81
LID - 10.1016/j.devcel.2009.09.001 [doi]
AB  - Dally-like (Dlp) is a glypican-type heparan sulfate proteoglycan (HSPG), 
      containing a protein core and attached glycosaminoglycan (GAG) chains. In 
      Drosophila wing discs, Dlp represses short-range Wingless (Wg) signaling, but 
      activates long-range Wg signaling. Here, we show that Dlp core protein has 
      similar biphasic activity as wild-type Dlp. Dlp core protein can interact with 
      Wg; the GAG chains enhance this interaction. Importantly, we find that Dlp 
      exhibits a biphasic response, regardless of whether its 
      glycosylphosphatidylinositol linkage to the membrane can be cleaved. Rather, the 
      transition from signaling activator to repressor is determined by the relative 
      expression levels of Dlp and the Wg receptor, Frizzled (Fz) 2. Based on these 
      data, we propose that the principal function of Dlp is to retain Wg on the cell 
      surface. As such, it can either compete with the receptor or provide ligands to 
      the receptor, depending on the ratios of Wg, Fz2, and Dlp.
FAU - Yan, Dong
AU  - Yan D
AD  - Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, 
      University of Cincinnati College of Medicine, Cincinnati, OH 45229, USA.
FAU - Wu, Yihui
AU  - Wu Y
FAU - Feng, Ying
AU  - Feng Y
FAU - Lin, Sheng-Cai
AU  - Lin SC
FAU - Lin, Xinhua
AU  - Lin X
LA  - eng
GR  - R01 GM063891/GM/NIGMS NIH HHS/United States
GR  - R01 GM063891-09/GM/NIGMS NIH HHS/United States
GR  - 2R01 GM063891/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Dev Cell
JT  - Developmental cell
JID - 101120028
RN  - 0 (Drosophila Proteins)
RN  - 0 (Frizzled Receptors)
RN  - 0 (Glycosaminoglycans)
RN  - 0 (Glycosylphosphatidylinositols)
RN  - 0 (Heparan Sulfate Proteoglycans)
RN  - 0 (Proteoglycans)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (Wnt1 Protein)
RN  - 0 (dlp protein, Drosophila)
RN  - 0 (fz2 protein, Drosophila)
RN  - 0 (wg protein, Drosophila)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - Animals
MH  - Drosophila Proteins/genetics/*metabolism
MH  - Drosophila melanogaster
MH  - Embryo, Nonmammalian/cytology/metabolism
MH  - Endocytosis
MH  - Frizzled Receptors/genetics/*metabolism
MH  - *Gene Expression Regulation, Developmental
MH  - Glycosaminoglycans/metabolism
MH  - Glycosylphosphatidylinositols/metabolism
MH  - Heparan Sulfate Proteoglycans/metabolism
MH  - Immunoprecipitation
MH  - Luciferases/metabolism
MH  - Proteoglycans/genetics/*metabolism
MH  - Receptors, G-Protein-Coupled/genetics/*metabolism
MH  - *Signal Transduction
MH  - Wings, Animal/cytology/metabolism
MH  - Wnt1 Protein/genetics/*metabolism
PMC - PMC3326419
MID - NIHMS365689
EDAT- 2009/10/27 06:00
MHDA- 2009/11/11 06:00
PMCR- 2012/04/15
CRDT- 2009/10/27 06:00
PHST- 2009/01/27 00:00 [received]
PHST- 2009/07/14 00:00 [revised]
PHST- 2009/09/01 00:00 [accepted]
PHST- 2009/10/27 06:00 [entrez]
PHST- 2009/10/27 06:00 [pubmed]
PHST- 2009/11/11 06:00 [medline]
PHST- 2012/04/15 00:00 [pmc-release]
AID - S1534-5807(09)00383-9 [pii]
AID - 10.1016/j.devcel.2009.09.001 [doi]
PST - ppublish
SO  - Dev Cell. 2009 Oct;17(4):470-81. doi: 10.1016/j.devcel.2009.09.001.