PMID- 19855381
OWN - NLM
STAT- MEDLINE
DCOM- 20100114
LR  - 20220409
IS  - 1529-2916 (Electronic)
IS  - 1529-2908 (Print)
IS  - 1529-2908 (Linking)
VI  - 11
IP  - 1
DP  - 2010 Jan
TI  - Enteric defensins are essential regulators of intestinal microbial ecology.
PG  - 76-83
LID - 10.1038/ni.1825 [doi]
AB  - Antimicrobial peptides are important effectors of innate immunity throughout the 
      plant and animal kingdoms. In the mammalian small intestine, Paneth cell 
      alpha-defensins are antimicrobial peptides that contribute to host defense 
      against enteric pathogens. To determine if alpha-defensins also govern intestinal 
      microbial ecology, we analyzed the intestinal microbiota of mice expressing a 
      human alpha-defensin gene (DEFA5) and in mice lacking an enzyme required for the 
      processing of mouse alpha-defensins. In these complementary models, we detected 
      significant alpha-defensin-dependent changes in microbiota composition, but not 
      in total bacterial numbers. Furthermore, DEFA5-expressing mice had striking 
      losses of segmented filamentous bacteria and fewer interleukin 17 
      (IL-17)-producing lamina propria T cells. Our data ascribe a new homeostatic role 
      to alpha-defensins in regulating the makeup of the commensal microbiota.
FAU - Salzman, Nita H
AU  - Salzman NH
AD  - Division of Gastroenterology, Medical College of Wisconsin, Milwaukee, Wisconsin, 
      USA. nsalzman@mcw.edu
FAU - Hung, Kuiechun
AU  - Hung K
FAU - Haribhai, Dipica
AU  - Haribhai D
FAU - Chu, Hiutung
AU  - Chu H
FAU - Karlsson-Sjoberg, Jenny
AU  - Karlsson-Sjoberg J
FAU - Amir, Elad
AU  - Amir E
FAU - Teggatz, Paul
AU  - Teggatz P
FAU - Barman, Melissa
AU  - Barman M
FAU - Hayward, Michael
AU  - Hayward M
FAU - Eastwood, Daniel
AU  - Eastwood D
FAU - Stoel, Maaike
AU  - Stoel M
FAU - Zhou, Yanjiao
AU  - Zhou Y
FAU - Sodergren, Erica
AU  - Sodergren E
FAU - Weinstock, George M
AU  - Weinstock GM
FAU - Bevins, Charles L
AU  - Bevins CL
FAU - Williams, Calvin B
AU  - Williams CB
FAU - Bos, Nicolaas A
AU  - Bos NA
LA  - eng
GR  - R01 AI050843-06/AI/NIAID NIH HHS/United States
GR  - R01 AI032738/AI/NIAID NIH HHS/United States
GR  - R01 AI032738-10/AI/NIAID NIH HHS/United States
GR  - AI/DK50843/AI/NIAID NIH HHS/United States
GR  - R01 AI085090/AI/NIAID NIH HHS/United States
GR  - AI32738/AI/NIAID NIH HHS/United States
GR  - R37 AI032738/AI/NIAID NIH HHS/United States
GR  - R01 AI057757/AI/NIAID NIH HHS/United States
GR  - R01 AI057757-05/AI/NIAID NIH HHS/United States
GR  - R21 AI057757/AI/NIAID NIH HHS/United States
GR  - R01 AI050843/AI/NIAID NIH HHS/United States
GR  - AI057757/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20091022
PL  - United States
TA  - Nat Immunol
JT  - Nature immunology
JID - 100941354
RN  - 0 (Interleukin-17)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (alpha-Defensins)
RN  - EC 3.4.24.23 (Matrix Metalloproteinase 7)
SB  - IM
CIN - Nat Immunol. 2010 Jan;11(1):49-50. PMID: 20016512
MH  - Animals
MH  - Bacteria/classification/genetics/growth & development
MH  - Colony Count, Microbial
MH  - *Ecology
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interleukin-17/immunology/metabolism
MH  - Intestinal Mucosa/*metabolism
MH  - Intestine, Small/immunology/metabolism/microbiology
MH  - Intestines/immunology/*microbiology
MH  - Male
MH  - Matrix Metalloproteinase 7/genetics/metabolism
MH  - Metagenome
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Mice, Knockout
MH  - Mice, Transgenic
MH  - Microscopy, Fluorescence
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - T-Lymphocytes, Helper-Inducer/cytology/immunology/metabolism
MH  - alpha-Defensins/genetics/immunology/*metabolism
PMC - PMC2795796
MID - NIHMS153160
COIS- Author Information The authors declare no competing financial interests.
EDAT- 2009/10/27 06:00
MHDA- 2010/01/15 06:00
PMCR- 2010/07/01
CRDT- 2009/10/27 06:00
PHST- 2009/08/24 00:00 [received]
PHST- 2009/10/16 00:00 [accepted]
PHST- 2009/10/27 06:00 [entrez]
PHST- 2009/10/27 06:00 [pubmed]
PHST- 2010/01/15 06:00 [medline]
PHST- 2010/07/01 00:00 [pmc-release]
AID - ni.1825 [pii]
AID - 10.1038/ni.1825 [doi]
PST - ppublish
SO  - Nat Immunol. 2010 Jan;11(1):76-83. doi: 10.1038/ni.1825. Epub 2009 Oct 22.