PMID- 19861998
OWN - NLM
STAT- MEDLINE
DCOM- 20091113
LR  - 20211020
IS  - 1532-1827 (Electronic)
IS  - 0007-0920 (Print)
IS  - 0007-0920 (Linking)
VI  - 101
IP  - 9
DP  - 2009 Nov 3
TI  - Intraepithelial CD8-positive T lymphocytes predict survival for patients with 
      serous stage III ovarian carcinomas: relevance of clonal selection of T 
      lymphocytes.
PG  - 1513-21
LID - 10.1038/sj.bjc.6605274 [doi]
AB  - BACKGROUND: The aim of this study was to investigate the prognostic effect of 
      tumour-infiltrating lymphocytes (TILs) in serous stage III ovarian carcinoma to 
      determine TIL clonality and to correlate this to Her2/neu expression. METHODS: 
      Formalin-fixed and paraffin-embedded ovarian carcinomas were examined for CD20-, 
      CD3-, CD4- and CD8-positive lymphocytes (n=100), and for Her2/neu-positive tumour 
      cells (n=55/100) by immunohistochemistry. Clonality analysis was carried out by 
      T-cell receptor gamma (TCRgamma) gene rearrangements (n=93/100). Statistical 
      analyses included experimental and clinico-pathological variables, as well as 
      disease-free (DFS) and overall (OS) survival. RESULTS: CD20-positive B 
      lymphocytes were present in 57.7% (stromal)/33.0% (intraepithelial) and 
      CD3-positive T lymphocytes in 99.0% (stromal)/90.2% (intraepithelial) of ovarian 
      carcinomas. Intraepithelial CD3-positive T lymphocytes were correlated with 
      improved DFS in optimally debulked patients (P=0.0402). Intraepithelial 
      CD8-positive T lymphocytes were correlated with improved OS in all optimally 
      debulked patients (P=0.0201) and in those undergoing paclitaxel/carboplatin 
      therapy (P=0.0092). Finally, rarified and clonal TCRgamma gene rearrangements 
      were detected in 37 out of 93 (39.8%) and 15 out of 93 (16.1%) cases, 
      respectively. This was marginally associated with improved DFS (P=0.0873). 
      Despite a significant correlation of HER2/neu status and intraepithelial 
      CD8-positive lymphocytes (P=0.0264), this was non-directional (R=-0.257; 
      P=0.0626). CONCLUSION: Improved survival of ovarian cancer patients is related to 
      the infiltration, clonal selection and intraepithelial persistence of T 
      lymphocytes.
FAU - Stumpf, M
AU  - Stumpf M
AD  - Department of Obstetrics and Gynecology, Albert-Ludwigs-University, Freiburg 
      79106, Germany.
FAU - Hasenburg, A
AU  - Hasenburg A
FAU - Riener, M-O
AU  - Riener MO
FAU - Jutting, U
AU  - Jutting U
FAU - Wang, C
AU  - Wang C
FAU - Shen, Y
AU  - Shen Y
FAU - Orlowska-Volk, M
AU  - Orlowska-Volk M
FAU - Fisch, P
AU  - Fisch P
FAU - Wang, Z
AU  - Wang Z
FAU - Gitsch, G
AU  - Gitsch G
FAU - Werner, M
AU  - Werner M
FAU - Lassmann, S
AU  - Lassmann S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090929
PL  - England
TA  - Br J Cancer
JT  - British journal of cancer
JID - 0370635
RN  - EC 2.7.10.1 (ERBB2 protein, human)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Female
MH  - Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor
MH  - Humans
MH  - Lymphocytes, Tumor-Infiltrating/*immunology
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Ovarian Neoplasms/*immunology/*mortality/pathology
MH  - Receptor, ErbB-2/analysis
PMC - PMC2778517
EDAT- 2009/10/29 06:00
MHDA- 2009/11/17 06:00
PMCR- 2010/11/03
CRDT- 2009/10/29 06:00
PHST- 2009/10/29 06:00 [entrez]
PHST- 2009/10/29 06:00 [pubmed]
PHST- 2009/11/17 06:00 [medline]
PHST- 2010/11/03 00:00 [pmc-release]
AID - 6605274 [pii]
AID - 10.1038/sj.bjc.6605274 [doi]
PST - ppublish
SO  - Br J Cancer. 2009 Nov 3;101(9):1513-21. doi: 10.1038/sj.bjc.6605274. Epub 2009 
      Sep 29.