PMID- 19866491
OWN - NLM
STAT- MEDLINE
DCOM- 20091110
LR  - 20221021
IS  - 1557-7422 (Electronic)
IS  - 1043-0342 (Linking)
VI  - 19
IP  - 11
DP  - 2008 Nov
TI  - Hyperthermia-induced targeting of thermosensitive gene carriers to tumors.
PG  - 1283-92
AB  - Locoregional hyperthermia (HT) can be used for site-directed activation of 
      macromolecular drug delivery systems. We have developed a gene delivery system 
      based on thermosensitive block copolymers (TSCs) with a phase transition 
      temperature of 42 degrees C [Zintchenko, A., Ogris, M., and Wagner, E. (2006). 
      Bioconjug. Chem. 17, 766-772], in which the statistical copolymer of 
      vinylpyrrolidinone and N-isopropylacryamide is grafted on polyethylenimine (PEI). 
      Here we applied polyplexes consisting of plasmid DNA and TSCs systemically in A/J 
      mice bearing a syngeneic Neuro2A neuroblastoma tumor subcutaneously in each hind 
      limb. One limb was selectively treated by HT at 42 degrees C, at the same time 
      that polyplexes were injected via the tail vein. Hyperthermia led to increased 
      accumulation of thermosensitive polymer and aggregation of thermosensitive 
      polyplexes in HT-treated tumors, resulting in up to 10-fold increased DNA 
      deposition compared with non-HT-treated tumor. The level of transgene expression 
      induced by TSC polyplexes in HT-treated tumors was significantly higher and 
      selective for tumor tissue. With nonthermosensitive PEI polyplexes HT did not 
      influence transgene deposition or expression in tumor.
FAU - Schwerdt, Alenka
AU  - Schwerdt A
AD  - Pharmaceutical Biology-Biotechnology, Department of Pharmacy, 
      Ludwig-Maximilians-Universitat, Munich, Germany.
FAU - Zintchenko, Arkadi
AU  - Zintchenko A
FAU - Concia, Massimo
AU  - Concia M
FAU - Roesen, Nick
AU  - Roesen N
FAU - Fisher, Kerry
AU  - Fisher K
FAU - Lindner, Lars H
AU  - Lindner LH
FAU - Issels, Rolf
AU  - Issels R
FAU - Wagner, Ernst
AU  - Wagner E
FAU - Ogris, Manfred
AU  - Ogris M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hum Gene Ther
JT  - Human gene therapy
JID - 9008950
RN  - 0 (Polymers)
RN  - 0 (RNA, Messenger)
RN  - 9002-98-6 (Polyethyleneimine)
RN  - 9007-49-2 (DNA)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - Animals
MH  - DNA/*administration & dosage
MH  - *Gene Transfer Techniques
MH  - *Genetic Therapy
MH  - Genetic Vectors
MH  - Hindlimb/metabolism/pathology
MH  - *Hyperthermia, Induced
MH  - Immunoenzyme Techniques
MH  - Luciferases/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred A
MH  - Neuroblastoma/genetics/*therapy
MH  - Polyethyleneimine/chemistry
MH  - Polymers/*chemistry
MH  - RNA, Messenger/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transfection
MH  - Transgenes/*physiology
EDAT- 2009/10/30 06:00
MHDA- 2009/11/11 06:00
CRDT- 2009/10/30 06:00
PHST- 2009/10/30 06:00 [entrez]
PHST- 2009/10/30 06:00 [pubmed]
PHST- 2009/11/11 06:00 [medline]
AID - 10.1089/hum.2008.064 [doi]
PST - ppublish
SO  - Hum Gene Ther. 2008 Nov;19(11):1283-92. doi: 10.1089/hum.2008.064.