PMID- 19898931
OWN - NLM
STAT- MEDLINE
DCOM- 20101026
LR  - 20220408
IS  - 1573-7217 (Electronic)
IS  - 0167-6806 (Print)
IS  - 0167-6806 (Linking)
VI  - 122
IP  - 3
DP  - 2010 Aug
TI  - Targeting breast stem cells with the cancer preventive compounds curcumin and 
      piperine.
PG  - 777-85
LID - 10.1007/s10549-009-0612-x [doi]
AB  - The cancer stem cell hypothesis asserts that malignancies arise in tissue stem 
      and/or progenitor cells through the dysregulation or acquisition of self-renewal. 
      In order to determine whether the dietary polyphenols, curcumin, and piperine are 
      able to modulate the self-renewal of normal and malignant breast stem cells, we 
      examined the effects of these compounds on mammosphere formation, expression of 
      the breast stem cell marker aldehyde dehydrogenase (ALDH), and Wnt signaling. 
      Mammosphere formation assays were performed after curcumin, piperine, and control 
      treatment in unsorted normal breast epithelial cells and normal stem and early 
      progenitor cells, selected by ALDH positivity. Wnt signaling was examined using a 
      Topflash assay. Both curcumin and piperine inhibited mammosphere formation, 
      serial passaging, and percent of ALDH+ cells by 50% at 5 microM and completely at 
      10 microM concentration in normal and malignant breast cells. There was no effect 
      on cellular differentiation. Wnt signaling was inhibited by both curcumin and 
      piperine by 50% at 5 microM and completely at 10 microM. Curcumin and piperine 
      separately, and in combination, inhibit breast stem cell self-renewal but do not 
      cause toxicity to differentiated cells. These compounds could be potential cancer 
      preventive agents. Mammosphere formation assays may be a quantifiable biomarker 
      to assess cancer preventive agent efficacy and Wnt signaling assessment can be a 
      mechanistic biomarker for use in human clinical trials.
FAU - Kakarala, Madhuri
AU  - Kakarala M
AD  - Division of Hematology/Oncology, Department of Internal Medicine and 
      Comprehensive Cancer Center, University of Michigan, 2150 Cancer Center, 1500 E. 
      Medical Center Dr., Ann Arbor, MI 48109, USA. mkakaral@umich.edu
FAU - Brenner, Dean E
AU  - Brenner DE
FAU - Korkaya, Hasan
AU  - Korkaya H
FAU - Cheng, Connie
AU  - Cheng C
FAU - Tazi, Karim
AU  - Tazi K
FAU - Ginestier, Christophe
AU  - Ginestier C
FAU - Liu, Suling
AU  - Liu S
FAU - Dontu, Gabriela
AU  - Dontu G
FAU - Wicha, Max S
AU  - Wicha MS
LA  - eng
GR  - R01 CA129765/CA/NCI NIH HHS/United States
GR  - R01 CA129765-02/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20091107
PL  - Netherlands
TA  - Breast Cancer Res Treat
JT  - Breast cancer research and treatment
JID - 8111104
RN  - 0 (Alkaloids)
RN  - 0 (Benzodioxoles)
RN  - 0 (Piperidines)
RN  - 0 (Polyunsaturated Alkamides)
RN  - 0 (Wnt Proteins)
RN  - EC 1.2.1.3 (Aldehyde Dehydrogenase)
RN  - IT942ZTH98 (Curcumin)
RN  - U71XL721QK (piperine)
SB  - IM
MH  - Aldehyde Dehydrogenase/metabolism
MH  - Alkaloids/administration & dosage
MH  - Antineoplastic Combined Chemotherapy Protocols/*therapeutic use
MH  - Benzodioxoles/administration & dosage
MH  - Breast/drug effects/metabolism/*pathology
MH  - Breast Neoplasms/*drug therapy/metabolism/pathology
MH  - Cell Differentiation/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Curcumin/administration & dosage
MH  - Female
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Neoplastic Stem Cells/*drug effects/metabolism/*pathology
MH  - Piperidines/administration & dosage
MH  - Polyunsaturated Alkamides/administration & dosage
MH  - Signal Transduction/drug effects
MH  - Wnt Proteins/metabolism
PMC - PMC3039120
MID - NIHMS172393
EDAT- 2009/11/10 06:00
MHDA- 2010/10/27 06:00
PMCR- 2011/08/01
CRDT- 2009/11/10 06:00
PHST- 2009/10/16 00:00 [received]
PHST- 2009/10/20 00:00 [accepted]
PHST- 2009/11/10 06:00 [entrez]
PHST- 2009/11/10 06:00 [pubmed]
PHST- 2010/10/27 06:00 [medline]
PHST- 2011/08/01 00:00 [pmc-release]
AID - 10.1007/s10549-009-0612-x [doi]
PST - ppublish
SO  - Breast Cancer Res Treat. 2010 Aug;122(3):777-85. doi: 10.1007/s10549-009-0612-x. 
      Epub 2009 Nov 7.