PMID- 19900439
OWN - NLM
STAT- MEDLINE
DCOM- 20100225
LR  - 20231213
IS  - 1095-564X (Electronic)
IS  - 0012-1606 (Print)
IS  - 0012-1606 (Linking)
VI  - 337
IP  - 2
DP  - 2010 Jan 15
TI  - Hippo signaling regulates Yorkie nuclear localization and activity through 14-3-3 
      dependent and independent mechanisms.
PG  - 303-12
LID - 10.1016/j.ydbio.2009.10.046 [doi]
AB  - The Hippo (Hpo) signaling pathway controls cell growth, proliferation and 
      apoptosis in both Drosophila and vertebrates. In Drosophila, Hpo signaling 
      regulates gene expression by inhibiting a transcription complex consisting of the 
      transcriptional coactivator Yorkie (Yki) and the TEAD/TEF family of transcription 
      factor Scalloped (Sd). Here we provide genetic evidence that both isoforms of 
      14-3-3, 14-3-3varepsilon and 14-3-3zeta, regulate Yki activity through modulating 
      its subcellular localization. Inactivation of 14-3-3 by RNAi or genetic mutations 
      enhanced whereas overexpression of 14-3-3 suppressed tissue overgrowth induced by 
      Yki overexpression. Loss of 14-3-3 resulted in the accumulation of Yki in the 
      nucleus. We found that regulation of Yki by 14-3-3 was mediated by 
      phosphorylation of Yki at S168. In addition, we found that Hpo signaling also 
      inhibited Yki nuclear localization and activity by phosphorylating Yki at S111 
      and S250, and this inhibition appears to be independent of 14-3-3. Finally, we 
      provided evidence that Hpo signaling restricted Yki nuclear localization 
      depending on CRM1-mediated nuclear export.
CI  - Copyright 2009 Elsevier Inc. All rights reserved.
FAU - Ren, Fangfang
AU  - Ren F
AD  - Department of Developmental Biology, University of Texas Southwestern Medical 
      Center, Dallas, TX 75390, USA.
FAU - Zhang, Lei
AU  - Zhang L
FAU - Jiang, Jin
AU  - Jiang J
LA  - eng
GR  - R01 GM067045/GM/NIGMS NIH HHS/United States
GR  - R01 GM067045-06/GM/NIGMS NIH HHS/United States
GR  - R01 GM061269-09/GM/NIGMS NIH HHS/United States
GR  - R01 GM067045-07/GM/NIGMS NIH HHS/United States
GR  - GM067045/GM/NIGMS NIH HHS/United States
GR  - GM061269/GM/NIGMS NIH HHS/United States
GR  - R01 GM061269-08/GM/NIGMS NIH HHS/United States
GR  - R01 GM061269/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20091106
PL  - United States
TA  - Dev Biol
JT  - Developmental biology
JID - 0372762
RN  - 0 (14-3-3 Proteins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Karyopherins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Trans-Activators)
RN  - 0 (YAP-Signaling Proteins)
RN  - 0 (Yki protein, Drosophila)
RN  - 17885-08-4 (Phosphoserine)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (hpo protein, Drosophila)
SB  - IM
MH  - 14-3-3 Proteins/*metabolism
MH  - Active Transport, Cell Nucleus
MH  - Animals
MH  - Cell Nucleus/*metabolism
MH  - Drosophila Proteins/antagonists & inhibitors/*metabolism
MH  - Drosophila melanogaster/cytology/growth & development/*metabolism
MH  - Eye/cytology/growth & development
MH  - Gene Knockdown Techniques
MH  - Intracellular Signaling Peptides and Proteins/*metabolism
MH  - Karyopherins/metabolism
MH  - Nuclear Proteins/antagonists & inhibitors/*metabolism
MH  - Phosphorylation
MH  - Phosphoserine/metabolism
MH  - Protein Isoforms/metabolism
MH  - Protein Serine-Threonine Kinases/*metabolism
MH  - Protein Transport
MH  - Receptors, Cytoplasmic and Nuclear/metabolism
MH  - *Signal Transduction
MH  - Subcellular Fractions/metabolism
MH  - Trans-Activators/antagonists & inhibitors/*metabolism
MH  - YAP-Signaling Proteins
MH  - Exportin 1 Protein
PMC - PMC2812623
MID - NIHMS158704
EDAT- 2009/11/11 06:00
MHDA- 2010/02/26 06:00
PMCR- 2011/01/15
CRDT- 2009/11/11 06:00
PHST- 2009/08/06 00:00 [received]
PHST- 2009/10/13 00:00 [revised]
PHST- 2009/10/31 00:00 [accepted]
PHST- 2009/11/11 06:00 [entrez]
PHST- 2009/11/11 06:00 [pubmed]
PHST- 2010/02/26 06:00 [medline]
PHST- 2011/01/15 00:00 [pmc-release]
AID - S0012-1606(09)01340-2 [pii]
AID - 10.1016/j.ydbio.2009.10.046 [doi]
PST - ppublish
SO  - Dev Biol. 2010 Jan 15;337(2):303-12. doi: 10.1016/j.ydbio.2009.10.046. Epub 2009 
      Nov 6.