PMID- 19903840
OWN - NLM
STAT- MEDLINE
DCOM- 20091221
LR  - 20220309
IS  - 1538-7445 (Electronic)
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 69
IP  - 23
DP  - 2009 Dec 1
TI  - Recognition and killing of brain tumor stem-like initiating cells by CD8+ 
      cytolytic T cells.
PG  - 8886-93
LID - 10.1158/0008-5472.CAN-09-2687 [doi]
AB  - Solid tumors contain a subset of stem-like cells that are resistant to the 
      cytotoxic effects of chemotherapy/radiotherapy, but their susceptibility to 
      cytolytic T lymphocyte (CTL) effector mechanisms has not been well characterized. 
      Using a panel of early-passage human brain tumor stem/initiating cell (BTSC) 
      lines derived from high-grade gliomas, we show that BTSCs are subject to 
      immunologic recognition and elimination by CD8(+) CTLs. Compared with 
      serum-differentiated CD133(low) tumor cells and established glioma cell lines, 
      BTSCs are equivalent with respect to expression levels of HLA class I and ICAM-1, 
      similar in their ability to trigger degranulation and cytokine synthesis by 
      antigen-specific CTLs, and equally susceptible to perforin-dependent CTL-mediated 
      cytolysis. BTSCs are also competent in the processing and presentation of 
      antigens as evidenced by the killing of these cells by CTL when antigen is 
      endogenously expressed. Moreover, we show that CTLs can eliminate all BTSCs with 
      tumor-initiating activity in an antigen-specific manner in vivo. Current models 
      predict that curative therapies for many cancers will require the elimination of 
      the stem/initiating population, and these studies lay the foundation for 
      developing immunotherapeutic approaches to eradicate this tumor population.
FAU - Brown, Christine E
AU  - Brown CE
AD  - Department of Cancer Immunotherapeutics, Division of Neurosurgery, City of Hope 
      National Medical Center, Duarte, California 91010, USA.
FAU - Starr, Renate
AU  - Starr R
FAU - Martinez, Catalina
AU  - Martinez C
FAU - Aguilar, Brenda
AU  - Aguilar B
FAU - D'Apuzzo, Massimo
AU  - D'Apuzzo M
FAU - Todorov, Ivan
AU  - Todorov I
FAU - Shih, Chu-Chih
AU  - Shih CC
FAU - Badie, Behnam
AU  - Badie B
FAU - Hudecek, Michael
AU  - Hudecek M
FAU - Riddell, Stanley R
AU  - Riddell SR
FAU - Jensen, Michael C
AU  - Jensen MC
LA  - eng
GR  - M01 RR000043-450856/RR/NCRR NIH HHS/United States
GR  - M01 RR000043-475806/RR/NCRR NIH HHS/United States
GR  - M01 RR000043-475779/RR/NCRR NIH HHS/United States
GR  - M01 RR000043-440856/RR/NCRR NIH HHS/United States
GR  - M01 RR00043/RR/NCRR NIH HHS/United States
GR  - P30 CA 33572/CA/NCI NIH HHS/United States
GR  - M01 RR000043/RR/NCRR NIH HHS/United States
GR  - P30 CA033572-200003/CA/NCI NIH HHS/United States
GR  - P30 CA033572/CA/NCI NIH HHS/United States
GR  - R01 CA103959/CA/NCI NIH HHS/United States
GR  - R01 CA103959-03/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20091110
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (AC133 Antigen)
RN  - 0 (Antigens, CD)
RN  - 0 (Glycoproteins)
RN  - 0 (HLA-A2 Antigen)
RN  - 0 (PROM1 protein, human)
RN  - 0 (Peptides)
RN  - 0 (Prom1 protein, mouse)
SB  - IM
MH  - AC133 Antigen
MH  - Animals
MH  - Antigen Presentation
MH  - Antigens, CD/biosynthesis/immunology
MH  - Brain Neoplasms/*immunology/pathology
MH  - Cell Death/immunology
MH  - Cell Line, Tumor
MH  - Glioma/*immunology/pathology
MH  - Glycoproteins/biosynthesis/immunology
MH  - HLA-A2 Antigen/immunology
MH  - Humans
MH  - Mice
MH  - Mice, Inbred NOD
MH  - Mice, SCID
MH  - Neoplastic Stem Cells/*immunology/pathology
MH  - Peptides/immunology
MH  - T-Lymphocytes, Cytotoxic/*immunology
MH  - Transplantation, Heterologous
PMC - PMC2789196
MID - NIHMS150326
EDAT- 2009/11/12 06:00
MHDA- 2009/12/22 06:00
PMCR- 2010/12/01
CRDT- 2009/11/12 06:00
PHST- 2009/11/12 06:00 [entrez]
PHST- 2009/11/12 06:00 [pubmed]
PHST- 2009/12/22 06:00 [medline]
PHST- 2010/12/01 00:00 [pmc-release]
AID - 0008-5472.CAN-09-2687 [pii]
AID - 10.1158/0008-5472.CAN-09-2687 [doi]
PST - ppublish
SO  - Cancer Res. 2009 Dec 1;69(23):8886-93. doi: 10.1158/0008-5472.CAN-09-2687. Epub 
      2009 Nov 10.