PMID- 19914372 OWN - NLM STAT- MEDLINE DCOM- 20100913 LR - 20240312 IS - 1090-2139 (Electronic) IS - 0889-1591 (Print) IS - 0889-1591 (Linking) VI - 24 IP - 5 DP - 2010 Jul TI - Brain dendritic cells in ischemic stroke: time course, activation state, and origin. PG - 724-37 LID - 10.1016/j.bbi.2009.11.002 [doi] AB - The immune response to stroke is comprised of inflammatory and regulatory processes. One cell type involved in both innate and adaptive immunity is the dendritic cell (DC). A DC population residing in the healthy brain (bDC) was identified using a transgenic mouse expressing enhanced yellow fluorescent protein (EYFP) under the promoter for the DC marker, CD11c (CD11c/EYFP Tg). To determine if bDC are involved in the immune response to cerebral ischemia, transient (40 min) middle cerebral artery occlusion (MCAO) followed by 6, 24, or 72 h reperfusion was conducted in CD11c/EYFP Tg mice. Our results demonstrated that DC accumulated in the ischemic hemisphere at 24 h post-MCAO-reperfusion, particularly in the border region of the infarct where T lymphocytes accrued. To distinguish resident bDC from the infiltrating peripheral DC, radiation chimeras [1. wild type (WT) hosts restored with CD11c/EYFP Tg bone marrow (BM) or 2. CD11c/EYFP Tg hosts restored with WT BM] were generated and examined by immunocytochemistry. These data confirmed that DC populating the core of the infarct at 72 h were of peripheral origin, whereas those in the border region were comprised primarily of resident bDC. The brain resident (CD45 intermediate) cells of CD11c/EYFP Tg mice were analyzed by flow cytometry. Compared to microglia, bDC displayed increased major histocompatibility class II (MHC II) and co-stimulatory molecules following MCAO-reperfusion. High levels of MHC II and the co-stimulatory molecule CD80 on bDC at 72 h corresponded to peak lymphocyte infiltration, and suggested a functional interaction between these two immune cell populations. CI - Copyright 2009 Elsevier Inc. All rights reserved. FAU - Felger, Jennifer C AU - Felger JC AD - Laboratory of Neuroendocrinology, The Rockefeller University, New York, NY 10065, USA. FAU - Abe, Takato AU - Abe T FAU - Kaunzner, Ulrike W AU - Kaunzner UW FAU - Gottfried-Blackmore, Andres AU - Gottfried-Blackmore A FAU - Gal-Toth, Judit AU - Gal-Toth J FAU - McEwen, Bruce S AU - McEwen BS FAU - Iadecola, Costantino AU - Iadecola C FAU - Bulloch, Karen AU - Bulloch K LA - eng GR - P01 AG016765-100001/AG/NIA NIH HHS/United States GR - R01 NS034179/NS/NINDS NIH HHS/United States GR - R01 NS035806/NS/NINDS NIH HHS/United States GR - NS35806/NS/NINDS NIH HHS/United States GR - R37 NS034179/NS/NINDS NIH HHS/United States GR - P01 AG016765/AG/NIA NIH HHS/United States GR - NS34179/NS/NINDS NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't DEP - 20091113 PL - Netherlands TA - Brain Behav Immun JT - Brain, behavior, and immunity JID - 8800478 RN - 0 (CD11c Antigen) SB - IM MH - Analysis of Variance MH - Animals MH - Brain/*immunology MH - Brain Ischemia/*immunology MH - CD11c Antigen/immunology MH - Dendritic Cells/*immunology MH - Flow Cytometry MH - Genes, MHC Class II/immunology MH - Immunohistochemistry MH - Leukocytes/immunology MH - Lymphocyte Activation/immunology MH - Mice MH - Mice, Transgenic MH - Microglia/immunology MH - Stroke/*immunology MH - T-Lymphocytes/immunology MH - Time Factors PMC - PMC2885548 MID - NIHMS165073 EDAT- 2009/11/17 06:00 MHDA- 2010/09/14 06:00 PMCR- 2011/07/01 CRDT- 2009/11/17 06:00 PHST- 2009/08/05 00:00 [received] PHST- 2009/11/04 00:00 [revised] PHST- 2009/11/05 00:00 [accepted] PHST- 2009/11/17 06:00 [entrez] PHST- 2009/11/17 06:00 [pubmed] PHST- 2010/09/14 06:00 [medline] PHST- 2011/07/01 00:00 [pmc-release] AID - S0889-1591(09)00513-3 [pii] AID - 10.1016/j.bbi.2009.11.002 [doi] PST - ppublish SO - Brain Behav Immun. 2010 Jul;24(5):724-37. doi: 10.1016/j.bbi.2009.11.002. Epub 2009 Nov 13.