PMID- 19940845
OWN - NLM
STAT- MEDLINE
DCOM- 20100429
LR  - 20230203
IS  - 1935-3456 (Electronic)
IS  - 1933-0219 (Print)
IS  - 1933-0219 (Linking)
VI  - 3
IP  - 2
DP  - 2010 Mar
TI  - Metagenomic analyses reveal antibiotic-induced temporal and spatial changes in 
      intestinal microbiota with associated alterations in immune cell homeostasis.
PG  - 148-58
LID - 10.1038/mi.2009.132 [doi]
AB  - Despite widespread use of antibiotics, few studies have measured their effects on 
      the burden or diversity of bacteria in the mammalian intestine. We developed an 
      oral antibiotic treatment protocol and characterized its effects on murine 
      intestinal bacterial communities and immune cell homeostasis. Antibiotic 
      administration resulted in a 10-fold reduction in the amount of intestinal 
      bacteria present and sequencing of 16S rDNA segments revealed significant 
      temporal and spatial effects on luminal and mucosal-associated communities 
      including reductions in luminal Firmicutes and mucosal-associated Lactobacillus 
      species, and persistence of bacteria belonging to the Bacteroidetes and 
      Proteobacteria phyla. Concurrently, antibiotic administration resulted in reduced 
      RELM beta production, and reduced production of interferon-gamma and 
      interleukin-17A by mucosal CD4(+) T lymphocytes. This comprehensive temporal and 
      spatial metagenomic analyses will provide a resource and framework to test the 
      influence of bacterial communities in murine models of human disease.
FAU - Hill, D A
AU  - Hill DA
AD  - Department of Pathobiology, University of Pennsylvania School of Veterinary 
      Medicine, Philadelphia, Pennsylvania, USA.
FAU - Hoffmann, C
AU  - Hoffmann C
FAU - Abt, M C
AU  - Abt MC
FAU - Du, Y
AU  - Du Y
FAU - Kobuley, D
AU  - Kobuley D
FAU - Kirn, T J
AU  - Kirn TJ
FAU - Bushman, F D
AU  - Bushman FD
FAU - Artis, D
AU  - Artis D
LA  - eng
GR  - R01 AI074878/AI/NIAID NIH HHS/United States
GR  - T32 AI060516-01A2/AI/NIAID NIH HHS/United States
GR  - DK50306/DK/NIDDK NIH HHS/United States
GR  - P30 DK050306/DK/NIDDK NIH HHS/United States
GR  - S10 RR024525/RR/NCRR NIH HHS/United States
GR  - R01 AI074878-01A1/AI/NIAID NIH HHS/United States
GR  - AI074878/AI/NIAID NIH HHS/United States
GR  - R21 AI083480-01/AI/NIAID NIH HHS/United States
GR  - T32 AI060516/AI/NIAID NIH HHS/United States
GR  - T32 AI05528/AI/NIAID NIH HHS/United States
GR  - R21 AI083480/AI/NIAID NIH HHS/United States
GR  - S10 RR024525-01/RR/NCRR NIH HHS/United States
GR  - R01 AI061570-01/AI/NIAID NIH HHS/United States
GR  - T32 AI055428/AI/NIAID NIH HHS/United States
GR  - R01 AI095466/AI/NIAID NIH HHS/United States
GR  - R01 AI061570/AI/NIAID NIH HHS/United States
GR  - T32 AI055428-01/AI/NIAID NIH HHS/United States
GR  - S10RR024525/RR/NCRR NIH HHS/United States
GR  - AI61570/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20091125
PL  - United States
TA  - Mucosal Immunol
JT  - Mucosal immunology
JID - 101299742
RN  - 0 (Anti-Bacterial Agents)
SB  - IM
MH  - Anti-Bacterial Agents/*pharmacology
MH  - Bacteria/*drug effects/genetics/immunology
MH  - *Biodiversity
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - Homeostasis/*drug effects
MH  - Humans
MH  - Intestines/drug effects/immunology/*microbiology
MH  - *Metagenomics
MH  - Reverse Transcriptase Polymerase Chain Reaction
PMC - PMC2824244
MID - NIHMS171081
EDAT- 2009/11/27 06:00
MHDA- 2010/04/30 06:00
PMCR- 2010/09/01
CRDT- 2009/11/27 06:00
PHST- 2009/11/27 06:00 [entrez]
PHST- 2009/11/27 06:00 [pubmed]
PHST- 2010/04/30 06:00 [medline]
PHST- 2010/09/01 00:00 [pmc-release]
AID - S1933-0219(22)01469-6 [pii]
AID - 10.1038/mi.2009.132 [doi]
PST - ppublish
SO  - Mucosal Immunol. 2010 Mar;3(2):148-58. doi: 10.1038/mi.2009.132. Epub 2009 Nov 
      25.