PMID- 19944676
OWN - NLM
STAT- MEDLINE
DCOM- 20100225
LR  - 20211020
IS  - 1095-564X (Electronic)
IS  - 0012-1606 (Print)
IS  - 0012-1606 (Linking)
VI  - 338
IP  - 1
DP  - 2010 Feb 1
TI  - miR-9a prevents apoptosis during wing development by repressing Drosophila 
      LIM-only.
PG  - 63-73
LID - 10.1016/j.ydbio.2009.11.025 [doi]
AB  - Loss of Drosophila mir-9a induces a subtle increase in sensory bristles, but a 
      substantial loss of wing tissue. Here, we establish that the latter phenotype is 
      largely due to ectopic apoptosis in the dorsal wing primordium, and we could 
      rescue wing development in the absence of this microRNA by dorsal-specific 
      inhibition of apoptosis. Such apoptosis was a consequence of de-repressing 
      Drosophila LIM-only (dLMO), which encodes a transcriptional regulator of wing and 
      neural development. We observed cell-autonomous elevation of endogenous dLMO and 
      a GFP-dLMO 3'UTR sensor in mir-9a mutant wing clones, and heterozygosity for dLMO 
      rescued the apoptosis and wing defects of mir-9a mutants. We also provide 
      evidence that dLMO, in addition to senseless, contributes to the bristle defects 
      of the mir-9a mutant. Unexpectedly, the upregulation of dLMO, loss of Cut, and 
      adult wing margin defects seen with mir-9a mutant clones were not recapitulated 
      by clonal loss of the miRNA biogenesis factors Dicer-1 or Pasha, even though 
      these mutant conditions similarly de-repressed miR-9a and dLMO sensor transgenes. 
      Therefore, the failure to observe a phenotype upon conditional knockout of a 
      miRNA processing factor does not reliably indicate the lack of critical roles of 
      miRNAs in a given setting.
FAU - Bejarano, Fernando
AU  - Bejarano F
AD  - Department of Developmental Biology, Sloan-Kettering Institute, 1275 York Ave, 
      Box 252, New York, NY 10065, USA.
FAU - Smibert, Peter
AU  - Smibert P
FAU - Lai, Eric C
AU  - Lai EC
LA  - eng
GR  - R01 GM083300/GM/NIGMS NIH HHS/United States
GR  - R01 GM083300-03/GM/NIGMS NIH HHS/United States
GR  - R01-GM083300/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20091126
PL  - United States
TA  - Dev Biol
JT  - Developmental biology
JID - 0372762
RN  - 0 (Bx protein, Drosophila)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (MIRN9 microRNA, Drosophila)
RN  - 0 (MicroRNAs)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Pasha protein, Drosophila)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (sens protein, Drosophila)
RN  - EC 2.7.7.- (Dcr-1 protein, Drosophila)
RN  - EC 3.1.26.3 (Ribonuclease III)
RN  - EC 3.6.4.13 (RNA Helicases)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Clone Cells
MH  - Drosophila Proteins/*metabolism
MH  - Drosophila melanogaster/*cytology/*embryology/enzymology
MH  - Heterozygote
MH  - Homeodomain Proteins/*metabolism
MH  - MicroRNAs/*metabolism
MH  - Mutation/genetics
MH  - Neurogenesis/genetics
MH  - Nuclear Proteins/metabolism
MH  - Phenotype
MH  - RNA Helicases/metabolism
MH  - RNA-Binding Proteins/metabolism
MH  - Ribonuclease III/metabolism
MH  - Sense Organs/metabolism/pathology
MH  - Transcription Factors/metabolism
MH  - Wings, Animal/*cytology/*embryology
PMC - PMC2812678
MID - NIHMS167304
EDAT- 2009/12/01 06:00
MHDA- 2010/02/26 06:00
PMCR- 2011/02/01
CRDT- 2009/12/01 06:00
PHST- 2009/08/20 00:00 [received]
PHST- 2009/11/16 00:00 [revised]
PHST- 2009/11/19 00:00 [accepted]
PHST- 2009/12/01 06:00 [entrez]
PHST- 2009/12/01 06:00 [pubmed]
PHST- 2010/02/26 06:00 [medline]
PHST- 2011/02/01 00:00 [pmc-release]
AID - S0012-1606(09)01380-3 [pii]
AID - 10.1016/j.ydbio.2009.11.025 [doi]
PST - ppublish
SO  - Dev Biol. 2010 Feb 1;338(1):63-73. doi: 10.1016/j.ydbio.2009.11.025. Epub 2009 
      Nov 26.