PMID- 1995093
OWN - NLM
STAT- MEDLINE
DCOM- 19910325
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 77
IP  - 5
DP  - 1991 Mar 1
TI  - NB4, a maturation inducible cell line with t(15;17) marker isolated from a human 
      acute promyelocytic leukemia (M3).
PG  - 1080-6
AB  - Acute promyelocytic leukemia (APL) is a well-defined entity among acute leukemia, 
      cytogenetically characterized by a t(15;17) (q22;q11-12) translocation. In vitro 
      and in vivo studies suggest that all-trans retinoic acid (RA) treatment restores 
      cell maturation. We have isolated the first permanent cell line with t(15;17), 
      derived from the marrow of a patient with APL in relapse. The establishment of 
      the cell line, its morphologic, karyotypic, and immunohistochemical features are 
      reported. RA induced cell line maturation. Cells strongly expressed myeloid 
      markers, but also some T-cell markers. Additional karyotypic abnormalities, a 12p 
      rearrangement and the possible presence of a homogeneous staining region (HSR) on 
      19q+ are discussed both in relation to T-cell (CD2, CD4) and monocyte (CD9) 
      markers, and to the acquired cell growth autonomy. The cell line represents a 
      remarkable tool for biomolecular studies.
FAU - Lanotte, M
AU  - Lanotte M
AD  - INSERM U-301, SDI No 15954.1 CNRS, Centre Hayem, Paris, France.
FAU - Martin-Thouvenin, V
AU  - Martin-Thouvenin V
FAU - Najman, S
AU  - Najman S
FAU - Balerini, P
AU  - Balerini P
FAU - Valensi, F
AU  - Valensi F
FAU - Berger, R
AU  - Berger R
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 5688UTC01R (Tretinoin)
SB  - IM
MH  - Adult
MH  - Antigens, CD/analysis
MH  - Antigens, Differentiation, Myelomonocytic/analysis
MH  - Cell Differentiation/drug effects
MH  - *Chromosomes, Human, Pair 15
MH  - *Chromosomes, Human, Pair 17
MH  - Female
MH  - Humans
MH  - Karyotyping
MH  - Leukemia, Promyelocytic, Acute/genetics/*pathology
MH  - Translocation, Genetic
MH  - Tretinoin/pharmacology
MH  - Tumor Cells, Cultured/*pathology
EDAT- 1991/03/01 00:00
MHDA- 1991/03/01 00:01
CRDT- 1991/03/01 00:00
PHST- 1991/03/01 00:00 [pubmed]
PHST- 1991/03/01 00:01 [medline]
PHST- 1991/03/01 00:00 [entrez]
AID - S0006-4971(20)84203-7 [pii]
PST - ppublish
SO  - Blood. 1991 Mar 1;77(5):1080-6.