PMID- 19951687
OWN - NLM
STAT- MEDLINE
DCOM- 20100604
LR  - 20220310
IS  - 1875-9777 (Electronic)
IS  - 1934-5909 (Print)
IS  - 1875-9777 (Linking)
VI  - 5
IP  - 6
DP  - 2009 Dec 4
TI  - Technical challenges in using human induced pluripotent stem cells to model 
      disease.
PG  - 584-95
LID - 10.1016/j.stem.2009.11.009 [doi]
AB  - Reprogramming of human somatic cells uses readily accessible tissue, such as skin 
      or blood, to generate embryonic-like induced pluripotent stem cells (iPSCs). This 
      procedure has been applied to somatic cells from patients who are classified into 
      a disease group, thus creating "disease-specific" iPSCs. Here, we examine the 
      challenges and assumptions in creating a disease model from a single cell of the 
      patient. Both the kinetics of disease onset and progression as well as the 
      spatial localization of disease in the patient's body are challenges to disease 
      modeling. New tools in genetic modification, reprogramming, biomaterials, and 
      animal models can be used for addressing these challenges.
FAU - Saha, Krishanu
AU  - Saha K
AD  - The Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.
FAU - Jaenisch, Rudolf
AU  - Jaenisch R
LA  - eng
GR  - R01-HDO45022/PHS HHS/United States
GR  - R01 CA087869-09/CA/NCI NIH HHS/United States
GR  - R01 HD045022-06/HD/NICHD NIH HHS/United States
GR  - R01 CA084198-10/CA/NCI NIH HHS/United States
GR  - R01-CA087869/CA/NCI NIH HHS/United States
GR  - R37-CA084198/CA/NCI NIH HHS/United States
GR  - R37 CA084198/CA/NCI NIH HHS/United States
GR  - R01 CA087869/CA/NCI NIH HHS/United States
GR  - R01 HD045022/HD/NICHD NIH HHS/United States
GR  - R01 CA084198/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - United States
TA  - Cell Stem Cell
JT  - Cell stem cell
JID - 101311472
SB  - IM
MH  - Animals
MH  - Cell Culture Techniques
MH  - Cell Differentiation
MH  - Disease Models, Animal
MH  - Genomics
MH  - Humans
MH  - Induced Pluripotent Stem Cells/metabolism/*pathology
PMC - PMC2921621
MID - NIHMS165809
EDAT- 2009/12/03 06:00
MHDA- 2010/06/05 06:00
PMCR- 2010/12/04
CRDT- 2009/12/03 06:00
PHST- 2009/12/03 06:00 [entrez]
PHST- 2009/12/03 06:00 [pubmed]
PHST- 2010/06/05 06:00 [medline]
PHST- 2010/12/04 00:00 [pmc-release]
AID - S1934-5909(09)00582-7 [pii]
AID - 10.1016/j.stem.2009.11.009 [doi]
PST - ppublish
SO  - Cell Stem Cell. 2009 Dec 4;5(6):584-95. doi: 10.1016/j.stem.2009.11.009.