PMID- 19996414
OWN - NLM
STAT- MEDLINE
DCOM- 20100503
LR  - 20210206
IS  - 1528-0020 (Electronic)
IS  - 0006-4971 (Linking)
VI  - 115
IP  - 13
DP  - 2010 Apr 1
TI  - Single-cell analysis of the common lymphoid progenitor compartment reveals 
      functional and molecular heterogeneity.
PG  - 2601-9
LID - 10.1182/blood-2009-08-236398 [doi]
AB  - To investigate molecular events involved in the regulation of lymphoid lineage 
      commitment, we crossed lambda5 reporter transgenic mice to Rag1-GFP knockin mice. 
      This allowed us to subfractionate common lymphoid progenitors and pre-pro-B 
      (fraction A) cells into lambda5(-)Rag1(low), lambda5(-)Rag1(high), and 
      lambda5(+)Rag1(high) cells. Clonal in vitro differentiation analysis demonstrated 
      that Rag1(low) cells gave rise to B/T and NK cells. Rag1(high) cells displayed 
      reduced NK-cell potential with preserved capacity to generate B- and T-lineage 
      cells, whereas the lambda5(+) cells were B-lineage restricted. Ebf1 and Pax5 
      expression was largely confined to the Rag1(high) populations. These cells also 
      expressed a higher level of the surface protein LY6D, providing an additional 
      tool for the analysis of early lymphoid development. These data suggest that the 
      classic common lymphoid progenitor compartment composes a mixture of cells with 
      relatively restricted lineage potentials, thus opening new possibilities to 
      investigate early hematopoiesis.
FAU - Mansson, Robert
AU  - Mansson R
AD  - Department for Clinical and Experimental Sciences, Linkoping University, 
      Linkoping, Sweden.
FAU - Zandi, Sasan
AU  - Zandi S
FAU - Welinder, Eva
AU  - Welinder E
FAU - Tsapogas, Panagiotis
AU  - Tsapogas P
FAU - Sakaguchi, Nobuo
AU  - Sakaguchi N
FAU - Bryder, David
AU  - Bryder D
FAU - Sigvardsson, Mikael
AU  - Sigvardsson M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091208
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, Ly)
RN  - 0 (Biomarkers)
RN  - 0 (Ebf1 protein, mouse)
RN  - 0 (GPI-Linked Proteins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Ly6d protein, mouse)
RN  - 0 (PAX5 Transcription Factor)
RN  - 0 (Pax5 protein, mouse)
RN  - 0 (Pou2af1 protein, mouse)
RN  - 0 (Trans-Activators)
RN  - 128559-51-3 (RAG-1 protein)
SB  - IM
CIN - Blood. 2010 Apr 1;115(13):2562-3. PMID: 20360473
MH  - Animals
MH  - Antigens, Ly/biosynthesis/genetics
MH  - Biomarkers
MH  - Cell Lineage
MH  - Cells, Cultured/cytology/metabolism
MH  - Coculture Techniques
MH  - Flow Cytometry
MH  - GPI-Linked Proteins
MH  - Gene Expression Profiling
MH  - Gene Knock-In Techniques
MH  - Genes, Reporter
MH  - Homeodomain Proteins/genetics
MH  - Killer Cells, Natural/cytology
MH  - Lymphocyte Subsets/cytology
MH  - Lymphocytes/*cytology/metabolism
MH  - Lymphopoiesis
MH  - Mice
MH  - Mice, Transgenic
MH  - PAX5 Transcription Factor/biosynthesis/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - Trans-Activators/biosynthesis/genetics
EDAT- 2009/12/10 06:00
MHDA- 2010/05/04 06:00
CRDT- 2009/12/10 06:00
PHST- 2009/12/10 06:00 [entrez]
PHST- 2009/12/10 06:00 [pubmed]
PHST- 2010/05/04 06:00 [medline]
AID - S0006-4971(20)56559-2 [pii]
AID - 10.1182/blood-2009-08-236398 [doi]
PST - ppublish
SO  - Blood. 2010 Apr 1;115(13):2601-9. doi: 10.1182/blood-2009-08-236398. Epub 2009 
      Dec 8.