PMID- 20010841
OWN - NLM
STAT- MEDLINE
DCOM- 20100302
LR  - 20211020
IS  - 1545-9985 (Electronic)
IS  - 1545-9985 (Linking)
VI  - 17
IP  - 1
DP  - 2010 Jan
TI  - A conserved activation element in BMP signaling during Drosophila development.
PG  - 69-76
LID - 10.1038/nsmb.1715 [doi]
AB  - The transforming growth factor beta (TGF-beta) family member Decapentaplegic 
      (Dpp) is a key regulator of patterning and growth in Drosophila development. 
      Previous studies have identified a short DNA motif called the silencer element 
      (SE), which recruits a trimeric Smad complex and the repressor Schnurri to 
      downregulate target enhancers upon Dpp signaling. We have now isolated the 
      minimal enhancer of the dad gene and discovered a short motif we termed the 
      activating element (AE). The AE is similar to the SE and recruits the Smad 
      proteins via a conserved mechanism. However, the AE and SE differ at important 
      nucleotide positions. As a consequence, the AE does not recruit Schnurri but 
      rather integrates repressive input by the default repressor Brinker and 
      activating input by the Smad signal transducers Mothers against Dpp (Mad) and 
      Medea via competitive DNA binding. The AE allows the identification of hitherto 
      unknown direct Dpp targets and is functionally conserved in vertebrates.
FAU - Weiss, Alexander
AU  - Weiss A
AD  - Biozentrum der Universitat Basel, Basel, Switzerland.
FAU - Charbonnier, Enrica
AU  - Charbonnier E
FAU - Ellertsdottir, Elin
AU  - Ellertsdottir E
FAU - Tsirigos, Aristotelis
AU  - Tsirigos A
FAU - Wolf, Christian
AU  - Wolf C
FAU - Schuh, Reinhard
AU  - Schuh R
FAU - Pyrowolakis, George
AU  - Pyrowolakis G
FAU - Affolter, Markus
AU  - Affolter M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091213
PL  - United States
TA  - Nat Struct Mol Biol
JT  - Nature structural & molecular biology
JID - 101186374
RN  - 0 (Dad protein, Drosophila)
RN  - 0 (Drosophila Proteins)
RN  - 0 (dpp protein, Drosophila)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Computational Biology
MH  - Drosophila/*embryology
MH  - Drosophila Proteins/*genetics/*metabolism
MH  - Electrophoretic Mobility Shift Assay
MH  - Enhancer Elements, Genetic/*genetics
MH  - Gene Expression Regulation, Developmental/*genetics
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Molecular Sequence Data
MH  - Sequence Analysis, DNA
MH  - Signal Transduction/*physiology
MH  - Silencer Elements, Transcriptional/genetics/physiology
MH  - Zebrafish/*embryology
EDAT- 2009/12/17 06:00
MHDA- 2010/03/03 06:00
CRDT- 2009/12/17 06:00
PHST- 2009/02/04 00:00 [received]
PHST- 2009/10/08 00:00 [accepted]
PHST- 2009/12/17 06:00 [entrez]
PHST- 2009/12/17 06:00 [pubmed]
PHST- 2010/03/03 06:00 [medline]
AID - nsmb.1715 [pii]
AID - 10.1038/nsmb.1715 [doi]
PST - ppublish
SO  - Nat Struct Mol Biol. 2010 Jan;17(1):69-76. doi: 10.1038/nsmb.1715. Epub 2009 Dec 
      13.