PMID- 20025999
OWN - NLM
STAT- MEDLINE
DCOM- 20100914
LR  - 20220331
IS  - 1878-7568 (Electronic)
IS  - 1742-7061 (Linking)
VI  - 6
IP  - 6
DP  - 2010 Jun
TI  - Synthesis and characterization of hyaluronic acid-poly(ethylene glycol) hydrogels 
      via Michael addition: An injectable biomaterial for cartilage repair.
PG  - 1968-77
LID - 10.1016/j.actbio.2009.12.024 [doi]
AB  - Injectable hydrogels based on hyaluronic acid (HA) and poly(ethylene glycol) 
      (PEG) were designed as biodegradable matrices for cartilage tissue engineering. 
      Solutions of HA conjugates containing thiol functional groups (HA-SH) and PEG 
      vinylsulfone (PEG-VS) macromers were cross-linked via Michael addition to form a 
      three-dimensional network under physiological conditions. Gelation times varied 
      from 14min to less than 1min, depending on the molecular weights of HA-SH and 
      PEG-VS, degree of substitution (DS) of HA-SH and total polymer concentration. 
      When the polymer concentration was increased from 2% to 6% (w/v) in the presence 
      of 100Uml(-1) hyaluronidase the degradation time increased from 3 to 15days. 
      Hydrogels with a homogeneous distribution of cells were obtained when 
      chondrocytes were mixed with the precursor solutions. Culturing cell-hydrogel 
      constructs prepared from HA185k-SH with a DS of 28 and cross-linked with 
      PEG5k-4VS for 3weeks in vitro revealed that the cells were viable and that cell 
      division took place. Gel-cell matrices degraded in approximately 3weeks, as shown 
      by a significant decrease in dry gel mass. At day 21 glycosaminoglycans and 
      collagen type II were found to have accumulated in hydrogels. These results 
      indicate that these injectable hydrogels have a high potential for cartilage 
      tissue engineering.
CI  - Copyright 2009 Acta Materialia Inc. Published by Elsevier Ltd. All rights 
      reserved.
FAU - Jin, R
AU  - Jin R
AD  - Department of Polymer Chemistry and Biomaterials, MIRA Institute for Biomedical 
      Technology and Technical Medicine, Faculty of Science and Technology, University 
      of Twente, PO Box 217, 7500 AE Enschede, The Netherlands.
FAU - Moreira Teixeira, L S
AU  - Moreira Teixeira LS
FAU - Krouwels, A
AU  - Krouwels A
FAU - Dijkstra, P J
AU  - Dijkstra PJ
FAU - van Blitterswijk, C A
AU  - van Blitterswijk CA
FAU - Karperien, M
AU  - Karperien M
FAU - Feijen, J
AU  - Feijen J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091216
PL  - England
TA  - Acta Biomater
JT  - Acta biomaterialia
JID - 101233144
RN  - 0 (Biocompatible Materials)
RN  - 0 (Hydrogels)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - 9004-61-9 (Hyaluronic Acid)
SB  - IM
MH  - Animals
MH  - Biocompatible Materials/*chemical synthesis
MH  - Cartilage, Articular/injuries/surgery
MH  - Cattle
MH  - Cells, Cultured
MH  - Chondrocytes/*cytology/*physiology
MH  - Hyaluronic Acid/*chemical synthesis
MH  - Hydrogels/administration & dosage/*chemical synthesis
MH  - Injections, Intra-Articular
MH  - Materials Testing
MH  - Polyethylene Glycols/*chemical synthesis
EDAT- 2009/12/23 06:00
MHDA- 2010/09/15 06:00
CRDT- 2009/12/23 06:00
PHST- 2009/07/14 00:00 [received]
PHST- 2009/11/12 00:00 [revised]
PHST- 2009/12/10 00:00 [accepted]
PHST- 2009/12/23 06:00 [entrez]
PHST- 2009/12/23 06:00 [pubmed]
PHST- 2010/09/15 06:00 [medline]
AID - S1742-7061(09)00565-0 [pii]
AID - 10.1016/j.actbio.2009.12.024 [doi]
PST - ppublish
SO  - Acta Biomater. 2010 Jun;6(6):1968-77. doi: 10.1016/j.actbio.2009.12.024. Epub 
      2009 Dec 16.