PMID- 20028757
OWN - NLM
STAT- MEDLINE
DCOM- 20100416
LR  - 20220408
IS  - 1557-3265 (Electronic)
IS  - 1078-0432 (Print)
IS  - 1078-0432 (Linking)
VI  - 16
IP  - 1
DP  - 2010 Jan 1
TI  - Aldehyde dehydrogenase 1-positive cancer stem cells mediate metastasis and poor 
      clinical outcome in inflammatory breast cancer.
PG  - 45-55
LID - 10.1158/1078-0432.CCR-09-1630 [doi]
AB  - PURPOSE: To examine the role of cancer stem cells (CSC) in mediating metastasis 
      in inflammatory breast cancer (IBC) and the association of these cells with 
      patient outcome in this aggressive type of breast cancer. EXPERIMENTAL DESIGN: 
      CSCs were isolated from SUM149 and MARY-X, an IBC cell line and primary 
      xenograft, by virtue of increased aldehyde dehydrogenase (ALDH) activity as 
      assessed by the ALDEFLUOR assay. Invasion and metastasis of CSC populations were 
      assessed by in vitro and mouse xenograft assays. Expression of ALDH1 was 
      determined on a retrospective series of 109 IBC patients and this was correlated 
      with histoclinical data. All statistical tests were two sided. Log-rank tests 
      using Kaplan-Meier analysis were used to determine the correlation of ALDH1 
      expression with development of metastasis and patient outcome. RESULTS: Both in 
      vitro and xenograft assays showed that invasion and metastasis in IBC are 
      mediated by a cellular component that displays ALDH activity. Furthermore, 
      expression of ALDH1 in IBC was an independent predictive factor for early 
      metastasis and decreased survival in this patient population. CONCLUSIONS: These 
      results suggest that the metastatic, aggressive behavior of IBC may be mediated 
      by a CSC component that displays ALDH enzymatic activity. ALDH1 expression 
      represents the first independent prognostic marker to predict metastasis and poor 
      patient outcome in IBC. The results illustrate how stem cell research can 
      translate into clinical practice in the IBC field.
FAU - Charafe-Jauffret, Emmanuelle
AU  - Charafe-Jauffret E
AD  - Department of Molecular Oncology, Institut Paoli-Calmettes/UMR891 Institut 
      National de la Sante et de la Recherche Medicale, Universite de la Mediterranee, 
      Marseille Cancer Research Center, Marseille, France. 
      jauffrete@marseille.fnclcc.fr
FAU - Ginestier, Christophe
AU  - Ginestier C
FAU - Iovino, Flora
AU  - Iovino F
FAU - Tarpin, Carole
AU  - Tarpin C
FAU - Diebel, Mark
AU  - Diebel M
FAU - Esterni, Benjamin
AU  - Esterni B
FAU - Houvenaeghel, Gilles
AU  - Houvenaeghel G
FAU - Extra, Jean-Marc
AU  - Extra JM
FAU - Bertucci, Francois
AU  - Bertucci F
FAU - Jacquemier, Jocelyne
AU  - Jacquemier J
FAU - Xerri, Luc
AU  - Xerri L
FAU - Dontu, Gabriela
AU  - Dontu G
FAU - Stassi, Giorgio
AU  - Stassi G
FAU - Xiao, Yi
AU  - Xiao Y
FAU - Barsky, Sanford H
AU  - Barsky SH
FAU - Birnbaum, Daniel
AU  - Birnbaum D
FAU - Viens, Patrice
AU  - Viens P
FAU - Wicha, Max S
AU  - Wicha MS
LA  - eng
GR  - P36CA45692/CA/NCI NIH HHS/United States
GR  - R01 CA101860-06/CA/NCI NIH HHS/United States
GR  - CA29765/CA/NCI NIH HHS/United States
GR  - R01 CA101860/CA/NCI NIH HHS/United States
GR  - CA101860/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20091222
PL  - United States
TA  - Clin Cancer Res
JT  - Clinical cancer research : an official journal of the American Association for 
      Cancer Research
JID - 9502500
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Isoenzymes)
RN  - EC 1.2.1 (Aldehyde Dehydrogenase 1 Family)
RN  - EC 1.2.1.3 (Aldehyde Dehydrogenase)
RN  - EC 1.2.1.36 (ALDH1A1 protein, human)
RN  - EC 1.2.1.36 (ALDH1A1 protein, mouse)
RN  - EC 1.2.1.36 (Retinal Dehydrogenase)
SB  - IM
MH  - Aldehyde Dehydrogenase/*metabolism
MH  - Aldehyde Dehydrogenase 1 Family
MH  - Animals
MH  - Biomarkers, Tumor/metabolism
MH  - Breast Neoplasms/*enzymology/mortality/pathology
MH  - Cell Line, Tumor
MH  - Female
MH  - Humans
MH  - Inflammation/*enzymology
MH  - Isoenzymes/*metabolism
MH  - Mice
MH  - Mice, Inbred NOD
MH  - Mice, SCID
MH  - *Neoplasm Metastasis
MH  - Neoplasm Transplantation
MH  - Neoplastic Stem Cells/*enzymology
MH  - Retinal Dehydrogenase
MH  - Treatment Outcome
PMC - PMC2874875
MID - NIHMS200775
EDAT- 2009/12/24 06:00
MHDA- 2010/04/17 06:00
PMCR- 2011/01/01
CRDT- 2009/12/24 06:00
PHST- 2009/12/24 06:00 [entrez]
PHST- 2009/12/24 06:00 [pubmed]
PHST- 2010/04/17 06:00 [medline]
PHST- 2011/01/01 00:00 [pmc-release]
AID - 1078-0432.CCR-09-1630 [pii]
AID - 10.1158/1078-0432.CCR-09-1630 [doi]
PST - ppublish
SO  - Clin Cancer Res. 2010 Jan 1;16(1):45-55. doi: 10.1158/1078-0432.CCR-09-1630. Epub 
      2009 Dec 22.