PMID- 20040089
OWN - NLM
STAT- MEDLINE
DCOM- 20100315
LR  - 20220309
IS  - 1471-2407 (Electronic)
IS  - 1471-2407 (Linking)
VI  - 9
DP  - 2009 Dec 29
TI  - A reproducible brain tumour model established from human glioblastoma biopsies.
PG  - 465
LID - 10.1186/1471-2407-9-465 [doi]
AB  - BACKGROUND: Establishing clinically relevant animal models of glioblastoma 
      multiforme (GBM) remains a challenge, and many commonly used cell line-based 
      models do not recapitulate the invasive growth patterns of patient GBMs. 
      Previously, we have reported the formation of highly invasive tumour xenografts 
      in nude rats from human GBMs. However, implementing tumour models based on 
      primary tissue requires that these models can be sufficiently standardised with 
      consistently high take rates. METHODS: In this work, we collected data on growth 
      kinetics from a material of 29 biopsies xenografted in nude rats, and 
      characterised this model with an emphasis on neuropathological and radiological 
      features. RESULTS: The tumour take rate for xenografted GBM biopsies were 96% and 
      remained close to 100% at subsequent passages in vivo, whereas only one of four 
      lower grade tumours engrafted. Average time from transplantation to the onset of 
      symptoms was 125 days +/- 11.5 SEM. Histologically, the primary xenografts 
      recapitulated the invasive features of the parent tumours while endothelial cell 
      proliferations and necrosis were mostly absent. After 4-5 in vivo passages, the 
      tumours became more vascular with necrotic areas, but also appeared more 
      circumscribed. MRI typically revealed changes related to tumour growth, several 
      months prior to the onset of symptoms. CONCLUSIONS: In vivo passaging of patient 
      GBM biopsies produced tumours representative of the patient tumours, with high 
      take rates and a reproducible disease course. The model provides combinations of 
      angiogenic and invasive phenotypes and represents a good alternative to in vitro 
      propagated cell lines for dissecting mechanisms of brain tumour progression.
FAU - Wang, Jian
AU  - Wang J
AD  - Department of Biomedicine, University of Bergen, N-5009 Bergen, Norway. 
      jian.wang@biomed.uib.no
FAU - Miletic, Hrvoje
AU  - Miletic H
FAU - Sakariassen, Per O
AU  - Sakariassen PO
FAU - Huszthy, Peter C
AU  - Huszthy PC
FAU - Jacobsen, Hege
AU  - Jacobsen H
FAU - Brekka, Narve
AU  - Brekka N
FAU - Li, Xingang
AU  - Li X
FAU - Zhao, Peng
AU  - Zhao P
FAU - Mork, Sverre
AU  - Mork S
FAU - Chekenya, Martha
AU  - Chekenya M
FAU - Bjerkvig, Rolf
AU  - Bjerkvig R
FAU - Enger, Per O
AU  - Enger PO
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091229
PL  - England
TA  - BMC Cancer
JT  - BMC cancer
JID - 100967800
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Animals
MH  - Biopsy
MH  - Brain Neoplasms/*pathology
MH  - *Disease Models, Animal
MH  - Female
MH  - Glioblastoma/*pathology
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Transplantation/pathology
MH  - Rats
MH  - *Rats, Nude
MH  - Reproducibility of Results
MH  - Transplantation, Heterologous
MH  - Tumor Cells, Cultured
MH  - Young Adult
PMC - PMC2810304
EDAT- 2009/12/31 06:00
MHDA- 2010/03/17 06:00
PMCR- 2009/12/29
CRDT- 2009/12/31 06:00
PHST- 2009/06/23 00:00 [received]
PHST- 2009/12/29 00:00 [accepted]
PHST- 2009/12/31 06:00 [entrez]
PHST- 2009/12/31 06:00 [pubmed]
PHST- 2010/03/17 06:00 [medline]
PHST- 2009/12/29 00:00 [pmc-release]
AID - 1471-2407-9-465 [pii]
AID - 10.1186/1471-2407-9-465 [doi]
PST - epublish
SO  - BMC Cancer. 2009 Dec 29;9:465. doi: 10.1186/1471-2407-9-465.