PMID- 20045355
OWN - NLM
STAT- MEDLINE
DCOM- 20100323
LR  - 20100215
IS  - 1097-4180 (Electronic)
IS  - 1074-7613 (Linking)
VI  - 32
IP  - 1
DP  - 2010 Jan 29
TI  - Thymoproteasome shapes immunocompetent repertoire of CD8+ T cells.
PG  - 29-40
LID - 10.1016/j.immuni.2009.10.009 [doi]
AB  - How self-peptides displayed in the thymus contribute to the development of 
      immunocompetent and self-protective T cells is largely unknown. In contrast, the 
      role of thymic self-peptides in eliminating self-reactive T cells and thereby 
      preventing autoimmunity is well established. A type of proteasome, termed 
      thymoproteasome, is specifically expressed by thymic cortical epithelial cells 
      (cTECs) and is required for the generation of optimal cellularity of CD8+ T 
      cells. Here, we show that cTECs displayed thymoproteasome-specific peptide-MHC 
      class I complexes essential for the positive selection of major and diverse 
      repertoire of MHC class I-restricted T cells. CD8+ T cells generated in the 
      absence of thymoproteasomes displayed a markedly altered T cell receptor 
      repertoire that was defective in both allogeneic and antiviral responses. These 
      results demonstrate that thymoproteasome-dependent self-peptide production is 
      required for the development of an immunocompetent repertoire of CD8+ T cells.
CI  - Copyright 2010 Elsevier Inc. All rights reserved.
FAU - Nitta, Takeshi
AU  - Nitta T
AD  - Division of Experimental Immunology, Institute for Genome Research, University of 
      Tokushima, Tokushima 770-8503, Japan.
FAU - Murata, Shigeo
AU  - Murata S
FAU - Sasaki, Katsuhiro
AU  - Sasaki K
FAU - Fujii, Hideki
AU  - Fujii H
FAU - Ripen, Adiratna Mat
AU  - Ripen AM
FAU - Ishimaru, Naozumi
AU  - Ishimaru N
FAU - Koyasu, Shigeo
AU  - Koyasu S
FAU - Tanaka, Keiji
AU  - Tanaka K
FAU - Takahama, Yousuke
AU  - Takahama Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091231
PL  - United States
TA  - Immunity
JT  - Immunity
JID - 9432918
RN  - 0 (Histocompatibility Antigens Class I)
RN  - EC 3.4.25.1 (Proteasome Endopeptidase Complex)
SB  - IM
MH  - Animals
MH  - CD8-Positive T-Lymphocytes/cytology/*immunology/metabolism
MH  - Cell Differentiation/*immunology
MH  - Epithelial Cells/*immunology/metabolism
MH  - Histocompatibility Antigens Class I/immunology
MH  - Lymphocyte Activation/immunology
MH  - Mice
MH  - Mice, Knockout
MH  - Proteasome Endopeptidase Complex/*immunology
MH  - Self Tolerance/immunology
MH  - T-Lymphocyte Subsets/cytology/*immunology/metabolism
MH  - Thymus Gland/cytology/immunology/metabolism
EDAT- 2010/01/05 06:00
MHDA- 2010/03/24 06:00
CRDT- 2010/01/05 06:00
PHST- 2009/06/16 00:00 [received]
PHST- 2009/10/03 00:00 [revised]
PHST- 2009/10/14 00:00 [accepted]
PHST- 2010/01/05 06:00 [entrez]
PHST- 2010/01/05 06:00 [pubmed]
PHST- 2010/03/24 06:00 [medline]
AID - S1074-7613(09)00543-3 [pii]
AID - 10.1016/j.immuni.2009.10.009 [doi]
PST - ppublish
SO  - Immunity. 2010 Jan 29;32(1):29-40. doi: 10.1016/j.immuni.2009.10.009. Epub 2009 
      Dec 31.