PMID- 20144789
OWN - NLM
STAT- MEDLINE
DCOM- 20100316
LR  - 20220317
IS  - 1875-9777 (Electronic)
IS  - 1875-9777 (Linking)
VI  - 6
IP  - 2
DP  - 2010 Feb 5
TI  - Spindle orientation bias in gut epithelial stem cell compartments is lost in 
      precancerous tissue.
PG  - 175-81
LID - 10.1016/j.stem.2009.12.007 [doi]
AB  - The importance of asymmetric divisions for stem cell function and maintenance is 
      well established in the developing nervous system and the skin; however, its role 
      in gut epithelium and its importance for tumorigenesis is still debated. We 
      demonstrate alignment of mitotic spindles perpendicular to the apical surface 
      specifically in the stem cell compartments of mouse and human intestine and 
      colon. This orientation correlates with the asymmetric retention of 
      label-retaining DNA. Both the preference for perpendicular spindle alignment and 
      asymmetric label retention are lost in precancerous tissue heterozygous for the 
      adenomatous polyposis coli tumor suppressor (Apc). This loss correlates with cell 
      shape changes specifically in the stem cell compartment. Our data suggest that 
      loss of asymmetric division in stem cells might contribute to the oncogenic 
      effect of Apc mutations in gut epithelium.
CI  - Copyright 2010 Elsevier Inc. All rights reserved.
FAU - Quyn, Aaron J
AU  - Quyn AJ
AD  - Cell and Developmental Biology, University of Dundee, Dundee, DD1 5EH, UK.
FAU - Appleton, Paul L
AU  - Appleton PL
FAU - Carey, Francis A
AU  - Carey FA
FAU - Steele, Robert J C
AU  - Steele RJ
FAU - Barker, Nick
AU  - Barker N
FAU - Clevers, Hans
AU  - Clevers H
FAU - Ridgway, Rachel A
AU  - Ridgway RA
FAU - Sansom, Owen J
AU  - Sansom OJ
FAU - Nathke, Inke S
AU  - Nathke IS
LA  - eng
GR  - 11243/CRUK_/Cancer Research UK/United Kingdom
GR  - A11243/CRUK_/Cancer Research UK/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell Stem Cell
JT  - Cell stem cell
JID - 101311472
RN  - 0 (Adenomatous Polyposis Coli Protein)
SB  - IM
CIN - Cell Stem Cell. 2010 Feb 5;6(2):91-2. doi: 10.1016/j.stem.2010.01.009. PMID: 
      20144779
CIN - Cell Stem Cell. 2010 Apr 2;6(4):298-299. doi: 10.1016/j.stem.2010.03.005. PMID: 
      20362533
MH  - Adenomatous Polyposis Coli Protein/genetics/metabolism
MH  - Animals
MH  - Cell Division
MH  - Cell Shape
MH  - Colon/*cytology/metabolism
MH  - Epithelial Cells/*cytology/metabolism
MH  - Humans
MH  - Intestinal Mucosa/metabolism
MH  - Intestines/*cytology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mutation
MH  - Precancerous Conditions/genetics/metabolism/*pathology
MH  - Spindle Apparatus/*metabolism
MH  - Stem Cells/*cytology/metabolism
EDAT- 2010/02/11 06:00
MHDA- 2010/03/17 06:00
CRDT- 2010/02/11 06:00
PHST- 2009/06/30 00:00 [received]
PHST- 2009/10/26 00:00 [revised]
PHST- 2009/12/15 00:00 [accepted]
PHST- 2010/02/11 06:00 [entrez]
PHST- 2010/02/11 06:00 [pubmed]
PHST- 2010/03/17 06:00 [medline]
AID - S1934-5909(09)00630-4 [pii]
AID - 10.1016/j.stem.2009.12.007 [doi]
PST - ppublish
SO  - Cell Stem Cell. 2010 Feb 5;6(2):175-81. doi: 10.1016/j.stem.2009.12.007.