PMID- 20150502
OWN - NLM
STAT- MEDLINE
DCOM- 20100309
LR  - 20211020
IS  - 1095-9203 (Electronic)
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 327
IP  - 5967
DP  - 2010 Feb 12
TI  - Vibrio cholerae VpsT regulates matrix production and motility by directly sensing 
      cyclic di-GMP.
PG  - 866-8
LID - 10.1126/science.1181185 [doi]
AB  - Microorganisms can switch from a planktonic, free-swimming life-style to a 
      sessile, colonial state, called a biofilm, which confers resistance to 
      environmental stress. Conversion between the motile and biofilm life-styles has 
      been attributed to increased levels of the prokaryotic second messenger cyclic 
      di-guanosine monophosphate (c-di-GMP), yet the signaling mechanisms mediating 
      such a global switch are poorly understood. Here we show that the transcriptional 
      regulator VpsT from Vibrio cholerae directly senses c-di-GMP to inversely control 
      extracellular matrix production and motility, which identifies VpsT as a master 
      regulator for biofilm formation. Rather than being regulated by phosphorylation, 
      VpsT undergoes a change in oligomerization on c-di-GMP binding.
FAU - Krasteva, Petya V
AU  - Krasteva PV
AD  - Department of Molecular Medicine, College of Veterinary Medicine, Cornell 
      University, Ithaca, NY 14853, USA.
FAU - Fong, Jiunn C N
AU  - Fong JC
FAU - Shikuma, Nicholas J
AU  - Shikuma NJ
FAU - Beyhan, Sinem
AU  - Beyhan S
FAU - Navarro, Marcos V A S
AU  - Navarro MV
FAU - Yildiz, Fitnat H
AU  - Yildiz FH
FAU - Sondermann, Holger
AU  - Sondermann H
LA  - eng
SI  - GEO/GSE19479
SI  - PDB/3KLN
SI  - PDB/3KLO
GR  - R01 AI055987-06A1/AI/NIAID NIH HHS/United States
GR  - 1R01GM081373/GM/NIGMS NIH HHS/United States
GR  - R01 GM081373/GM/NIGMS NIH HHS/United States
GR  - P30 EB009998/EB/NIBIB NIH HHS/United States
GR  - R01 GM081373-03/GM/NIGMS NIH HHS/United States
GR  - R01 AI055987/AI/NIAID NIH HHS/United States
GR  - R01AI055987/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Polysaccharides, Bacterial)
RN  - 0 (Transcription Factors)
RN  - 61093-23-0 (bis(3',5')-cyclic diguanylic acid)
RN  - H2D2X058MU (Cyclic GMP)
SB  - IM
MH  - Amino Acid Motifs
MH  - Bacterial Proteins/chemistry/genetics/*metabolism
MH  - Binding Sites
MH  - Biofilms/*growth & development
MH  - Crystallography, X-Ray
MH  - Cyclic GMP/*analogs & derivatives/metabolism
MH  - DNA, Bacterial/metabolism
MH  - Dimerization
MH  - Extracellular Matrix/*metabolism
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Bacterial
MH  - Models, Molecular
MH  - Movement
MH  - Point Mutation
MH  - Polysaccharides, Bacterial/genetics/metabolism
MH  - Protein Folding
MH  - Protein Multimerization
MH  - Protein Structure, Tertiary
MH  - Signal Transduction
MH  - Transcription Factors/chemistry/genetics/*metabolism
MH  - Transcription, Genetic
MH  - Vibrio cholerae O1/cytology/genetics/*physiology
PMC - PMC2828054
MID - NIHMS175521
EDAT- 2010/02/13 06:00
MHDA- 2010/03/10 06:00
PMCR- 2011/02/12
CRDT- 2010/02/13 06:00
PHST- 2010/02/13 06:00 [entrez]
PHST- 2010/02/13 06:00 [pubmed]
PHST- 2010/03/10 06:00 [medline]
PHST- 2011/02/12 00:00 [pmc-release]
AID - 327/5967/866 [pii]
AID - 10.1126/science.1181185 [doi]
PST - ppublish
SO  - Science. 2010 Feb 12;327(5967):866-8. doi: 10.1126/science.1181185.