PMID- 2015403
OWN - NLM
STAT- MEDLINE
DCOM- 19910521
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 77
IP  - 8
DP  - 1991 Apr 15
TI  - Two-step differentiation of AML-193 leukemic line: terminal maturation is induced 
      by positive interaction of retinoic acid with granulocyte colony-stimulating 
      factor (CSF) and vitamin D3 with monocyte CSF.
PG  - 1804-12
AB  - The human AML-193 cell line requires exogenous granulocyte-monocyte 
      colony-stimulating factor (GM-CSF) or interleukin-3 (IL-3) for growth in liquid 
      or semisolid medium. However, these CSFs do not stimulate the differentiation of 
      the cell line. We show that addition of all-trans retinoic acid (RA) or 1,25 
      dihydroxyvitamin D3 (D3) induces AML-193 cells to differentiate into the 
      granulocytic or monocytic lineage, respectively. On the other hand, addition of 
      either G- or M-CSF alone exerts virtually no differentiative effect. Terminal 
      granulocytic or monocytic differentiation was observed when AML-193 cells were 
      treated with RA and G-CSF, or D3 and M-CSF, respectively, as evaluated by cell 
      morphology, analysis of surface antigens, and phagocytic functions. These 
      positive interactions indicate that the differentiating activity of G- and M-CSF 
      on leukemic cells may be unmasked by preliminary treatment with RA and D3, 
      respectively, ie, the physiologic inducers override the leukemic differentiation 
      blockade and CFSs exert their differentiative activity on the unblocked leukemic 
      cells. These preliminary observations on a single cell line may pave the way for 
      the designing of clinical protocols combining physiologic inducer(s) and 
      hematopoietic growth factor(s) in the treatment of acute leukemia.
FAU - Valtieri, M
AU  - Valtieri M
AD  - Department of Hematology-Oncology, Istituto Superiore di Sanita, Rome, Italy.
FAU - Boccoli, G
AU  - Boccoli G
FAU - Testa, U
AU  - Testa U
FAU - Barletta, C
AU  - Barletta C
FAU - Peschle, C
AU  - Peschle C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, CD)
RN  - 0 (Cytokines)
RN  - 0 (Recombinant Proteins)
RN  - 5688UTC01R (Tretinoin)
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - FXC9231JVH (Calcitriol)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Antigens, CD/analysis
MH  - Calcitriol/*pharmacology
MH  - Cell Differentiation/*drug effects
MH  - Cell Line
MH  - Clone Cells
MH  - Cytokines/*pharmacology
MH  - Drug Interactions
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/*pharmacology
MH  - Humans
MH  - Kinetics
MH  - Leukemia, Myeloid, Acute
MH  - Macrophage Colony-Stimulating Factor/*pharmacology
MH  - Recombinant Proteins/pharmacology
MH  - Tretinoin/*pharmacology
EDAT- 1991/04/15 00:00
MHDA- 1991/04/15 00:01
CRDT- 1991/04/15 00:00
PHST- 1991/04/15 00:00 [pubmed]
PHST- 1991/04/15 00:01 [medline]
PHST- 1991/04/15 00:00 [entrez]
AID - S0006-4971(20)81581-X [pii]
PST - ppublish
SO  - Blood. 1991 Apr 15;77(8):1804-12.