PMID- 2017172
OWN - NLM
STAT- MEDLINE
DCOM- 19910521
LR  - 20220408
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 11
IP  - 5
DP  - 1991 May
TI  - Evi-1, a murine zinc finger proto-oncogene, encodes a sequence-specific 
      DNA-binding protein.
PG  - 2665-74
AB  - Evi-1 was originally identified as a common site of viral integration in murine 
      myeloid tumors. Evi-1 encodes a 120-kDa polypeptide containing 10 zinc finger 
      motifs located in two domains 380 amino acids apart and an acidic domain located 
      carboxy terminal to the second set of zinc fingers. These features suggest that 
      Evi-1 is a site-specific DNA-binding protein involved in the regulation of RNA 
      transcription. We have purified Evi-1 protein from E. coli and have employed a 
      gel shift-polymerase chain reaction method using random oligonucleotides to 
      identify a high-affinity binding site for Evi-1. The consensus sequence for this 
      binding site is TGACAAGATAA. Evi-1 protein specifically protects this motif from 
      DNase I digestion. By searching the nucleotide sequence data bases, we have found 
      this binding site both in sequences 5' to genes in putative or known regulatory 
      regions and within intron sequences.
FAU - Perkins, A S
AU  - Perkins AS
AD  - Mammalian Genetics Laboratory, NCI-Frederick Cancer Research and Development 
      Center, Maryland 21702.
FAU - Fishel, R
AU  - Fishel R
FAU - Jenkins, N A
AU  - Jenkins NA
FAU - Copeland, N G
AU  - Copeland NG
LA  - eng
GR  - N01-CO-74101/CO/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (MDS1 and EVI1 Complex Locus Protein)
RN  - 0 (Mecom protein, mouse)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Transcription Factors)
SB  - IM
GS  - Evi-1
MH  - Animals
MH  - Base Sequence
MH  - Binding Sites
MH  - Blotting, Western
MH  - Cloning, Molecular
MH  - DNA-Binding Proteins/*genetics/isolation & purification
MH  - Escherichia coli/genetics
MH  - MDS1 and EVI1 Complex Locus Protein
MH  - Mice
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Oligonucleotide Probes
MH  - Polymerase Chain Reaction/methods
MH  - *Proto-Oncogenes
MH  - Recombinant Fusion Proteins/isolation & purification
MH  - Sequence Homology, Nucleic Acid
MH  - *Transcription Factors
MH  - Zinc Fingers/*genetics
PMC - PMC360036
EDAT- 1991/05/01 00:00
MHDA- 1991/05/01 00:01
PMCR- 1991/05/01
CRDT- 1991/05/01 00:00
PHST- 1991/05/01 00:00 [pubmed]
PHST- 1991/05/01 00:01 [medline]
PHST- 1991/05/01 00:00 [entrez]
PHST- 1991/05/01 00:00 [pmc-release]
AID - 10.1128/mcb.11.5.2665-2674.1991 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1991 May;11(5):2665-74. doi: 10.1128/mcb.11.5.2665-2674.1991.